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PMID: 15319478 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A putative polyketide synthase/peptide synthetase from Magnaporthe grisea signals pathogen attack to resistant rice.

The Plant cell ·Vol. 16 ·No. 9 ·2004-09-00 ·Pages 2499-513

Böhnert HU, Fudal I, Dioh W, Tharreau D, Notteghem JL, Lebrun MH

Abstract

Isolates of the rice blast fungus Magnaporthe grisea that carry the gene encoding Avirulence Conferring Enzyme1 (ACE1) are specifically recognized by rice (Oryza sativa) cultivars carrying the resistance gene Pi33. This recognition enables resistant plants to activate a defense response. ACE1 was isolated by map-based cloning and encodes a putative hybrid between a polyketide synthase and a nonribosomal peptide synthetase, enzymes involved in microbial secondary metabolism. ACE1 is expressed exclusively during fungal penetration of host leaves, the time point at which plant defense reactions are triggered. Ace1 appears to be localized in the cytoplasm of the appressorium. Mutation of the putative catalytic site of the beta-ketoacyl synthase domain of Ace1 abolishes recognition of the fungus by resistant rice. This suggests that Ace1 biosynthetic activity is required for avirulence. Our results are consistent with the hypothesis that the fungal signal recognized by resistant rice plants is the secondary metabolite whose synthesis depends on Ace1.

MeSH Terms
Amino Acid Sequence/genetics Base Sequence/genetics Cytoplasm/enzymology,genetics DNA, Complementary/analysis,genetics Fungal Proteins/genetics,isolation & purification,metabolism Gene Expression Regulation, Fungal/genetics Immunity, Innate/genetics Macromolecular Substances Magnaporthe/enzymology,genetics Molecular Sequence Data Oryza/enzymology,genetics,microbiology Peptide Synthases/genetics,isolation & purification,metabolism Phylogeny Plant Diseases/genetics Plant Leaves/enzymology,genetics Polyketide Synthases/genetics,isolation & purification,metabolism Recombinant Fusion Proteins/genetics,metabolism Signal Transduction/genetics
Chemicals
DNA, Complementary Fungal Proteins Macromolecular Substances Recombinant Fusion Proteins Polyketide Synthases Peptide Synthases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Böhnert Heidi U
FRE 2579 Centre National de la Recherche Scientifique/Bayer CropScience, F-69263 Lyon Cedex 09, France.
Fudal Isabelle
Dioh Waly
Tharreau Didier
Notteghem Jean-Loup
Lebrun Marc-Henri
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.104.022715
Published
2004-09-00
Epub
2004-00-19
Pages
2499-513
Language
English
Region
England
NLM ID
9208688
PMCID
PMC520948
Subset
IM
Databases
GENBANK
AJ704622, AJ704623, BN000505, BN000506, BN000507
External Links
PubMed source DOI: 10.1105/tpc.104.022715 PMC Full Text View journal details
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