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PMID: 15282547 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

MicroRNA control of PHABULOSA in leaf development: importance of pairing to the microRNA 5' region.

The EMBO journal ·Vol. 23 ·No. 16 ·2004-08-18 ·Pages 3356-64

Mallory AC, Reinhart BJ, Jones-Rhoades MW, Tang G, Zamore PD, Barton MK, Bartel DP

Abstract

MicroRNAs (miRNAs) are approximately 22-nucleotide noncoding RNAs that can regulate gene expression by directing mRNA degradation or inhibiting productive translation. Dominant mutations in PHABULOSA (PHB) and PHAVOLUTA (PHV) map to a miR165/166 complementary site and impair miRNA-guided cleavage of these mRNAs in vitro. Here, we confirm that disrupted miRNA pairing, not changes in PHB protein sequence, causes the developmental defects in phb-d mutants. In planta, disrupting miRNA pairing near the center of the miRNA complementary site had far milder developmental consequences than more distal mismatches. These differences correlated with differences in miRNA-directed cleavage efficiency in vitro, where mismatch scanning revealed more tolerance for mismatches at the center and 3' end of the miRNA compared to mismatches to the miRNA 5' region. In this respect, miR165/166 resembles animal miRNAs in its pairing requirements. Pairing to the 5' portion of the small silencing RNA appears crucial regardless of the mode of post-transcriptional repression or whether it occurs in plants or animals, supporting a model in which this region of the silencing RNA nucleates pairing to its target.

MeSH Terms
Alleles Amino Acid Sequence Animals Arabidopsis/genetics,growth & development Arabidopsis Proteins/genetics Base Pairing Base Sequence Gene Expression Regulation, Plant/genetics Homeodomain Proteins/genetics MicroRNAs/genetics,metabolism Mutation/genetics Phenotype Plant Leaves/genetics,growth & development Plants, Genetically Modified RNA, Messenger/genetics,metabolism RNA, Plant/genetics,metabolism
Chemicals
Arabidopsis Proteins Homeodomain Proteins MicroRNAs PHB protein, Arabidopsis RNA, Messenger RNA, Plant
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Mallory Allison C
Whitehead Institute for Biomedical Research, Cambridge, MA 02142-1479, USA.
Reinhart Brenda J
Jones-Rhoades Matthew W
Tang Guiliang
Zamore Phillip D
Barton M Kathryn
Bartel David P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7600340
Published
2004-08-18
Epub
2004-00-29
Pages
3356-64
Language
English
Region
England
NLM ID
8208664
PMCID
PMC514513
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.emboj.7600340 PMC Full Text View journal details
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