Abstract
The localization and elasticity of connectin (titin) filaments in skinned fibres of frog skeletal muscle were examined for changes in the localization of connectin and in resting tension during partial depolymerization of thick filaments with a relaxing solution containing increased KCl concentrations. Immunoelectron microscopic studies revealed that deposites of antibodies against connectin at a sarcomere length of 3.0 microns remained at about 0.8 microns from the M-line, until the thick filament was depolymerized to the length of approximately 0.4 microns. On further depolymerization, the bound antibodies were found to move towards the Z-line and, on complete depolymerization, were observed to be within 0.3 microns of the Z-line; a marked decrease in resting tension accompanied this further depolymerization. These results suggest that connectin filament starts from the Z-line, extends to the M-line, and contributes to resting tension. After partial depolymerization of thick filaments, the distances between the anti-connectin deposits and the Z-line and between anti-connectin deposits and the M-line increased with sarcomere length, suggesting that connectin filaments are elastic along their entire length.
MeSH Terms
Actin Cytoskeleton/chemistry,drug effects,ultrastructure
Animals
Antibodies, Monoclonal/immunology
Connectin
Elasticity
Hypertonic Solutions/pharmacology
Microscopy, Immunoelectron
Models, Biological
Muscle Proteins/analysis,immunology
Muscle Tonus/physiology
Polymers
Potassium Chloride/pharmacology
Protein Kinases
Rana catesbeiana
Stress, Mechanical
Chemicals
Antibodies, Monoclonal
Connectin
Hypertonic Solutions
Muscle Proteins
Polymers
Potassium Chloride
Protein Kinases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Higuchi H
Department of Physiology, Jikei University School of Medicine, Tokyo, Japan.
Suzuki T
Kimura S
Yoshioka T
Maruyama K
Umazume Y
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