Home LiteratureArticle Details
PMID: 15272074 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Centromeric DNA sequences in the pathogenic yeast Candida albicans are all different and unique.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 101 ·No. 31 ·2004-08-03 ·Pages 11374-9

Sanyal K, Baum M, Carbon J

Abstract

In an approach to clone and characterize centromeric DNA sequences of Candida albicans by chromatin immunoprecipitation, we have used antibodies directed against an evolutionarily conserved histone H3-like protein, CaCse4p (CENP-A homolog). Sequence analysis of clones obtained by this procedure reveals that only eight relatively small regions (approximately 3 kb each) of the Can. albicans genome are selectively enriched. These CaCse4-bound sequences are located within 4- to 18-kb regions lacking ORFs and occur once in each of the eight chromosomes of Can. albicans. Binding of another evolutionarily conserved kinetochore protein, CaMif2p (CENP-C homolog), colocalizes with CaCse4p. Deletion of the CaCse4p-binding region of chromosome 7 results in a high rate of loss of the altered chromosome, confirming that CaCse4p, a centromeric histone in the CENP-A family, indeed identifies the functional centromeric DNA of Can. albicans. The CaCse4p-rich regions not only lack conserved DNA motifs of point (<400 bp) centromeres and repeated elements of regional (>40 kb) centromeres, but also each chromosome of Can. albicans contains a different and unique CaCse4p-rich centromeric DNA sequence, a centromeric property previously unobserved in other organisms.

MeSH Terms
Amino Acid Sequence Candida albicans/genetics,pathogenicity Centromere/genetics Chromosomal Proteins, Non-Histone/genetics,metabolism Chromosome Segregation Chromosomes, Fungal/physiology Conserved Sequence DNA, Fungal/genetics Evolution, Molecular Fungal Proteins/genetics,metabolism Molecular Sequence Data Precipitin Tests Protein Binding Virulence
Chemicals
Chromosomal Proteins, Non-Histone Cse4 protein, Candida albicans DNA, Fungal Fungal Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sanyal Kaustuv
Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, CA 93106, USA.
Baum Mary
Carbon John
References (40)
40 references, click to expand
  1. Molecular structure of a functional Drosophila centromere.
    Cell. 1997 Dec 26;91(7):1007-19 PMID: 9428523
  2. Whole genome PCR: application to the identification of sequences bound by gene regulatory proteins.
    Nucleic Acids Res. 1989 May 25;17(10):3645-53 PMID: 2734098
  3. Direct cloning of DNA that interacts in vivo with a specific protein: application to RNA polymerase II and sites of pausing in Drosophila.
    Nucleic Acids Res. 1998 Feb 15;26(4):919-24 PMID: 9461448
  4. Repetitive sequences (RPSs) in the chromosomes of Candida albicans are sandwiched between two novel stretches, HOK and RB2, common to each chromosome.
    Microbiology. 1998 Apr;144 ( Pt 4):849-57 PMID: 9579060
  5. Centromeres: proteins, protein complexes, and repeated domains at centromeres of simple eukaryotes.
    Curr Opin Genet Dev. 1998 Apr;8(2):212-8 PMID: 9610412
  6. A physical map of chromosome 7 of Candida albicans.
    Genetics. 1998 Aug;149(4):1739-52 PMID: 9691033
  7. Cse4p is a component of the core centromere of Saccharomyces cerevisiae.
    Cell. 1998 Sep 4;94(5):607-13 PMID: 9741625
  8. Identification of a mating type-like locus in the asexual pathogenic yeast Candida albicans.
    Science. 1999 Aug 20;285(5431):1271-5 PMID: 10455055
  9. Appearance and properties of L-sorbose-utilizing mutants of Candida albicans obtained on a selective plate.
    Genetics. 1999 Oct;153(2):653-64 PMID: 10511546
  10. In vivo cross-linking and immunoprecipitation for studying dynamic Protein:DNA associations in a chromatin environment.
    Methods. 1999 Nov;19(3):425-33 PMID: 10579938
  11. High-frequency occurrence of chromosome translocation in a mutant strain of Candida albicans by a suppressor mutation of ploidy shift.
    Yeast. 2000 Mar 30;16(5):411-22 PMID: 10705370
  12. Mapping chromosomal proteins in vivo by formaldehyde-crosslinked-chromatin immunoprecipitation.
    Trends Biochem Sci. 2000 Mar;25(3):99-104 PMID: 10694875
  13. Requirement of Mis6 centromere connector for localizing a CENP-A-like protein in fission yeast.
    Science. 2000 Jun 23;288(5474):2215-9 PMID: 10864871
  14. The plant kinetochore.
    Trends Plant Sci. 2000 Dec;5(12):543-7 PMID: 11120477
  15. A low copy number central sequence with strict symmetry and unusual chromatin structure in fission yeast centromere.
    Mol Biol Cell. 1992 Jul;3(7):819-35 PMID: 1515677
  16. Transcriptional silencing in yeast is associated with reduced nucleosome acetylation.
    Genes Dev. 1993 Apr;7(4):592-604 PMID: 8458576
  17. Centromeres of the fission yeast Schizosaccharomyces pombe are highly variable genetic loci.
    Mol Cell Biol. 1993 Aug;13(8):4578-87 PMID: 8336703
  18. Construction of an SfiI macrorestriction map of the Candida albicans genome.
    J Bacteriol. 1993 Oct;175(20):6637-51 PMID: 8407841
  19. CENP-C is required for maintaining proper kinetochore size and for a timely transition to anaphase.
    J Cell Biol. 1994 May;125(3):531-45 PMID: 8175879
  20. Chromosomal alterations of Candida albicans are associated with the gain and loss of assimilating functions.
    J Bacteriol. 1994 Jun;176(11):3231-41 PMID: 8195078
  21. A novel epigenetic effect can alter centromere function in fission yeast.
    Cell. 1994 Dec 2;79(5):865-74 PMID: 8001123
  22. The centromeric K-type repeat and the central core are together sufficient to establish a functional Schizosaccharomyces pombe centromere.
    Mol Biol Cell. 1994 Jul;5(7):747-61 PMID: 7812044
  23. The centromere enhancer mediates centromere activation in Schizosaccharomyces pombe.
    Mol Cell Biol. 1997 Jun;17(6):3305-14 PMID: 9154829
  24. Sequence and promoter regulation of the PCK1 gene encoding phosphoenolpyruvate carboxykinase of the fungal pathogen Candida albicans.
    Gene. 1997 Jun 19;192(2):235-40 PMID: 9224895
  25. Efficient conditional mutation of the vertebrate CENP-C gene.
    Hum Mol Genet. 1997 Dec;6(13):2301-8 PMID: 9361037
  26. A 330 kb CENP-A binding domain and altered replication timing at a human neocentromere.
    EMBO J. 2001 Apr 17;20(8):2087-96 PMID: 11296241
  27. HCP-4, a CENP-C-like protein in Caenorhabditis elegans, is required for resolution of sister centromeres.
    J Cell Biol. 2001 Jun 11;153(6):1199-208 PMID: 11402064
  28. Functional analysis of kinetochore assembly in Caenorhabditis elegans.
    J Cell Biol. 2001 Jun 11;153(6):1209-26 PMID: 11402065
  29. Determining centromere identity: cyclical stories and forking paths.
    Nat Rev Genet. 2001 Aug;2(8):584-96 PMID: 11483983
  30. The centromere paradox: stable inheritance with rapidly evolving DNA.
    Science. 2001 Aug 10;293(5532):1098-102 PMID: 11498581
  31. Centromeres and variant histones: what, where, when and why?
    Curr Opin Cell Biol. 2002 Jun;14(3):279-85 PMID: 12067649
  32. The CENP-A homolog CaCse4p in the pathogenic yeast Candida albicans is a centromere protein essential for chromosome transmission.
    Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12969-74 PMID: 12271118
  33. Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling.
    Cell. 2003 Feb 21;112(4):407-21 PMID: 12600307
  34. CENP-B interacts with CENP-C domains containing Mif2 regions responsible for centromere localization.
    J Biol Chem. 2004 Feb 13;279(7):5934-46 PMID: 14612452
  35. Human centromere repositioning "in progress".
    Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6542-7 PMID: 15084747
  36. The diploid genome sequence of Candida albicans.
    Proc Natl Acad Sci U S A. 2004 May 11;101(19):7329-34 PMID: 15123810
  37. Isolation of a yeast centromere and construction of functional small circular chromosomes.
    Nature. 1980 Oct 9;287(5782):504-9 PMID: 6999364
  38. Genomic substitutions of centromeres in Saccharomyces cerevisiae.
    Nature. 1983 Sep 1-7;305(5929):23-8 PMID: 6350891
  39. Transformation of yeast spheroplasts without cell fusion.
    Anal Biochem. 1987 Jun;163(2):391-7 PMID: 3310730
  40. Targeted disruption of mouse centromere protein C gene leads to mitotic disarray and early embryo death.
    Proc Natl Acad Sci U S A. 1998 Feb 3;95(3):1136-41 PMID: 9448298
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0404318101
Published
2004-08-03
Epub
2004-00-22
Pages
11374-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC509209
Subset
IM
Grants
NCI NIH HHS · R01 CA011034 · United States
NCI NIH HHS · CA-11034 · United States
External Links
PubMed source DOI: 10.1073/pnas.0404318101 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2