Home LiteratureArticle Details
PMID: 15259059 Published · ppublish English Journal Article

Expression of cyclooxygenase-2 in human esophageal squamous cell carcinomas.

World journal of gastroenterology ·Vol. 10 ·No. 15 ·2004-08-01 ·Pages 2168-73

Jiang JG, Tang JB, Chen CL, Liu BX, Fu XN, Zhu ZH, Qu W, Cianflone K, Waalkes MP, Wang DW

Abstract

To determine whether cyclooxygenase-2 (COX-2) was expressed in human esophageal squamous cell carcinoma. Quantitative reverse transcription-polymerase chain reaction (RT-PCR), western blotting, immunohistoc-hemistry and immunofluorescence were used to assess the expression level of COX-2 in esophageal tissue. COX-2 mRNA levels were increased by >80-fold in esophageal squamous cell carcinoma when compared to adjacent noncancerous tissue. COX-2 protein was present in 21 of 30 cases of esophageal squamous cell carcinoma tissues, but was undetectable in noncancerous tissue. Immunohistochemistry was performed to directly show expression of COX-2 in tumor tissue. These results suggest that COX-2 may be an important factor for esophageal cancer and inhibition of COX-2 may be helpful for prevention and possibly treatment of this cancer.

MeSH Terms
Adult Aged Blotting, Western Carcinoma, Squamous Cell/metabolism Cyclooxygenase 2 Esophageal Neoplasms/metabolism Fluorescent Antibody Technique Humans Immunohistochemistry Isoenzymes/genetics,metabolism Membrane Proteins Middle Aged Prostaglandin-Endoperoxide Synthases/genetics,metabolism RNA, Messenger/metabolism Reverse Transcriptase Polymerase Chain Reaction
Chemicals
Isoenzymes Membrane Proteins RNA, Messenger Cyclooxygenase 2 PTGS2 protein, human Prostaglandin-Endoperoxide Synthases
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Jiang Jian-Gang
Department of Internal Medicine and Gene Therapy Center, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. dwwang@tjh.tjmu.edu.cn
Tang Jiang-Bo
Chen Chun-Lian
Liu Bao-Xing
Fu Xiang-Ning
Zhu Zhi-Hui
Qu Wei
Cianflone Katherine
Waalkes Michael-P
Wang Dao-Wen
References (45)
45 references, click to expand
  1. Sulindac in familial adenomatous polyposis.
    Lancet. 1993 Oct 30;342(8879):1120 PMID: 8105347
  2. Cyclooxygenase-2 expression in human colon cancer cells increases metastatic potential.
    Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3336-40 PMID: 9096394
  3. Human cyclooxygenase-2 cDNA.
    Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7384-8 PMID: 1380156
  4. Prostaglandin H synthases, nonsteroidal anti-inflammatory drugs, and colon cancer.
    FASEB J. 1997 Mar;11(4):234-47 PMID: 9068612
  5. Cloning two isoforms of rat cyclooxygenase: differential regulation of their expression.
    Arch Biochem Biophys. 1993 Dec;307(2):361-8 PMID: 8274023
  6. Expression of cyclooxygenase-1 and -2 in human colorectal cancer.
    Cancer Res. 1995 Sep 1;55(17):3785-9 PMID: 7641194
  7. Enhanced secretion of prostaglandin E2 by tissue-fixed macrophages in colonic carcinoma.
    Digestion. 1990;47(3):160-6 PMID: 1964656
  8. Cloning and expression of rat prostaglandin endoperoxide synthase (cyclooxygenase)-2 cDNA.
    Biochem Biophys Res Commun. 1993 Dec 15;197(2):494-500 PMID: 7916614
  9. Cloning and characterization of a growth factor-inducible cyclooxygenase gene from rat intestinal epithelial cells.
    Am J Physiol. 1994 May;266(5 Pt 1):G822-7 PMID: 8203528
  10. Chemopreventive activity of celecoxib, a specific cyclooxygenase-2 inhibitor, against colon carcinogenesis.
    Cancer Res. 1998 Feb 1;58(3):409-12 PMID: 9458081
  11. Prostaglandin endoperoxide synthase: why two isoforms?
    Am J Physiol. 1996 Mar;270(3 Pt 1):G393-400 PMID: 8638704
  12. Histopathologic effects of neoadjuvant therapies for advanced squamous cell carcinoma of the esophagus: multivariate analysis of predictive factors and p53 overexpression.
    Dis Esophagus. 2002;15(1):61-6 PMID: 12060045
  13. Further studies on the therapeutic effect of indomethacin on esophageal tumors.
    Cancer. 1986 Sep 1;58(5):1029-31 PMID: 3731036
  14. Epidemiology and risk factors in pancreatic cancer.
    Semin Oncol. 1996 Apr;23(2):241-50 PMID: 8623060
  15. Aspirin and the risk of colorectal cancer in women.
    N Engl J Med. 1995 Sep 7;333(10):609-14 PMID: 7637720
  16. Aspirin and reduced risk of esophageal carcinoma.
    Cancer. 1995 Oct 1;76(7):1116-9 PMID: 8630885
  17. Evaluation of pharmacological profile of meloxicam as an anti-inflammatory agent, with particular reference to its relative selectivity for cyclooxygenase-2 over cyclooxygenase-1.
    Pharmacology. 1997 Jul;55(1):44-53 PMID: 9309800
  18. Self-promotion? Intimate connections between APC and prostaglandin H synthase-2.
    Cell. 1996 Nov 29;87(5):783-6 PMID: 8945503
  19. Selected eicosanoids increase the proliferation rate of human colon carcinoma cell lines and mouse colonocytes in vivo.
    Biochim Biophys Acta. 1995 Sep 14;1258(2):215-23 PMID: 7548186
  20. Increased expression of cyclooxygenase 2 occurs frequently in human lung cancers, specifically in adenocarcinomas.
    Cancer Res. 1998 Sep 1;58(17):3761-4 PMID: 9731479
  21. Gene amplification profiling of esophageal squamous cell carcinomas by DNA array CGH.
    Biochem Biophys Res Commun. 2002 Aug 9;296(1):152-5 PMID: 12147242
  22. Prostaglandin H synthase and xenobiotic oxidation.
    Annu Rev Pharmacol Toxicol. 1990;30:1-45 PMID: 2111654
  23. Expression of cyclooxygenase-2 in human gastric carcinoma.
    Cancer Res. 1997 Apr 1;57(7):1276-80 PMID: 9102213
  24. Transcription of cyclooxygenase-2 is enhanced in transformed mammary epithelial cells.
    Cancer Res. 1996 Oct 1;56(19):4424-9 PMID: 8813136
  25. Antitumoral activity of indomethacin on experimental esophageal tumors.
    J Natl Cancer Inst. 1984 Mar;72(3):705-7 PMID: 6583454
  26. Treatment of colonic and rectal adenomas with sulindac in familial adenomatous polyposis.
    N Engl J Med. 1993 May 6;328(18):1313-6 PMID: 8385741
  27. Mechanistic studies on the selective inhibition of cyclooxygenase-2 by indanone derivatives.
    Biochem Pharmacol. 1996 Feb 9;51(3):285-90 PMID: 8573195
  28. Aspirin use and lung, colon, and breast cancer incidence in a prospective study.
    Epidemiology. 1994 Mar;5(2):138-46 PMID: 8172988
  29. Reduced risk of large-bowel adenomas among aspirin users. The Polyp Prevention Study Group.
    J Natl Cancer Inst. 1993 Jun 2;85(11):912-6 PMID: 8492320
  30. Sulindac causes regression of rectal polyps in familial adenomatous polyposis.
    Gastroenterology. 1991 Sep;101(3):635-9 PMID: 1650315
  31. Effects of nonsteroidal anti-inflammatory drugs on proliferation and on induction of apoptosis in colon cancer cells by a prostaglandin-independent pathway.
    Biochem Pharmacol. 1996 Jul 26;52(2):237-45 PMID: 8694848
  32. Effects of various prostaglandin synthesis inhibitors on pancreatic carcinogenesis in hamsters after initiation with N-nitrosobis(2-oxopropyl)amine.
    Carcinogenesis. 1990 Mar;11(3):393-5 PMID: 2311181
  33. Up-regulation of cyclooxygenase 2 gene expression in human colorectal adenomas and adenocarcinomas.
    Gastroenterology. 1994 Oct;107(4):1183-8 PMID: 7926468
  34. Pharmacology of prostaglandin endoperoxide synthase isozymes-1 and -2.
    Ann N Y Acad Sci. 1994 Apr 18;714:136-42 PMID: 8017762
  35. Lipopolysaccharide induces prostaglandin H synthase-2 protein and mRNA in human alveolar macrophages and blood monocytes.
    J Clin Invest. 1994 Jan;93(1):391-6 PMID: 8282809
  36. Suppression of azoxymethane-induced aberrant crypt foci in rat colon by nimesulide, a selective inhibitor of cyclooxygenase 2.
    J Cancer Res Clin Oncol. 1996;122(4):219-22 PMID: 8601574
  37. Aspirin and the potential role of prostaglandins in colon cancer.
    Cancer Res. 1992 Oct 15;52(20):5575-89 PMID: 1394181
  38. Cyclooxygenase gene expression in inflammation and angiogenesis.
    Ann N Y Acad Sci. 1993 Nov 30;696:197-204 PMID: 7509130
  39. Prostaglandins from tumours of human large bowel.
    Br J Cancer. 1977 Jun;35(6):881-4 PMID: 871372
  40. Aspirin use and risk of fatal cancer.
    Cancer Res. 1993 Mar 15;53(6):1322-7 PMID: 8443812
  41. Localization and expression of p27KIP1 in multistage colorectal carcinogenesis.
    Cancer Res. 1998 Jan 1;58(1):114-22 PMID: 9426067
  42. Increased expression of inducible nitric oxide synthase and cyclooxygenase-2 in Barrett's esophagus and associated adenocarcinomas.
    Cancer Res. 1998 Jul 15;58(14):2929-34 PMID: 9679948
  43. Expression and selective inhibition of the constitutive and inducible forms of human cyclo-oxygenase.
    Biochem J. 1995 Jan 15;305 ( Pt 2):479-84 PMID: 7832763
  44. Aspirin use and reduced risk of fatal colon cancer.
    N Engl J Med. 1991 Dec 5;325(23):1593-6 PMID: 1669840
  45. Evaluation of cyclooxygenase-2 inhibitor for potential chemopreventive properties in colon carcinogenesis.
    Cancer Res. 1996 Oct 15;56(20):4566-9 PMID: 8840961
Article Info
Journal
World journal of gastroenterology
Abbr.
World J Gastroenterol
ISSN
1007-9327
DOI
10.3748/wjg.v10.i15.2168
Email
dwwang@tjh.tjmu.edu.cn
Published
2004-08-01
Pages
2168-73
Language
English
Region
United States
NLM ID
100883448
PMCID
PMC4724986
Subset
IM
External Links
PubMed source DOI: 10.3748/wjg.v10.i15.2168 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2