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PMID: 15251435 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Histone deacetylase inhibitors regulate p21WAF1 gene expression at the post-transcriptional level in HepG2 cells.

FEBS letters ·Vol. 570 ·No. 1-3 ·2004-07-16 ·Pages 37-40

Hirsch CL, Bonham K

Abstract

Histone deacetylase inhibitors (HDIs) are thought to act primarily at the level of transcription inducing cell cycle arrest, differentiation and/or apoptosis in many cancer cell types. Induction of the potent cdk/cyclin inhibitor p21WAF1 is a key feature of this HDI mediated transcriptional re-programming phenomenon. However, in the current study we report that HDIs are also capable of inducing p21WAF1 through purely post-transcriptional events, namely increased mRNA stability. These studies highlight our growing appreciation for the complexities of HDI mediated effects and challenge our preconceptions regarding the action of these promising anti-neoplastics.

MeSH Terms

Apoptosis Blotting, Northern Cell Differentiation Cell Division Cell Line Chloramphenicol O-Acetyltransferase/metabolism Cyclin-Dependent Kinase Inhibitor p21 Cyclins/metabolism Dose-Response Relationship, Drug Enzyme Inhibitors/pharmacology Gene Expression Regulation Histone Deacetylase Inhibitors Histones/metabolism Humans Immunoblotting Luciferases/metabolism Plasmids/metabolism Promoter Regions, Genetic RNA/metabolism RNA, Messenger/metabolism Time Factors Transcription, Genetic Transcriptional Activation Transfection

Chemicals

CDKN1A protein, human Cyclin-Dependent Kinase Inhibitor p21 Cyclins Enzyme Inhibitors Histone Deacetylase Inhibitors Histones RNA, Messenger RNA Luciferases Chloramphenicol O-Acetyltransferase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hirsch Calley L

Department of Biochemistry, University of Saskatchewan, Saskatoon SK, Canada S7N 5E5.

Bonham Keith

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/j.febslet.2004.06.018

Published

2004-07-16

Pages

37-40

Language

English

Region

England

NLM ID

0155157

Subset

External Links

PubMed source DOI: 10.1016/j.febslet.2004.06.018 View journal details

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Histone deacetylase inhibitors regulate p21WAF1 gene expression at the post-transcriptional level in HepG2 cells.

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