Home LiteratureArticle Details
该文献已被撤稿（Retracted Publication），引用前请核实。
PMID: 15231814 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Retracted Publication

Transcriptional analysis of the groES-groEL1, groEL2, and dnaK genes in Corynebacterium glutamicum: characterization of heat shock-induced promoters.

Journal of bacteriology ·Vol. 186 ·No. 14 ·2004-07-00 ·Pages 4813-7

Barreiro C, González-Lavado E, Pátek M, Martín JF

Abstract

The appropriate conditions to switch on the heat shock promoters in Corynebacterium glutamicum were defined by Northern blot analysis. Transcriptional patterns were characterized for the groEL2 gene and the groES-groEL1 and dnaK operons. Transcriptional start points of these genes were determined by primer extension analysis, allowing the identification of CIRCE and HAIR boxes close to the -10 and -35 regions of the promoters. The presence of both CIRCE and HAIR sequences within a single promoter (P-groEL2) in bacteria is described for the first time. In addition, the dnaK promoter showed -10 and -35 sequences similar to those recognized by SigH of Mycobacterium and SigR of Streptomyces close to a second transcription start region with -10 and -35 boxes typical of promoters for housekeeping genes.

MeSH Terms
Adaptation, Physiological Bacterial Proteins/genetics Chaperonin 10/biosynthesis,genetics,physiology Chaperonin 60/biosynthesis,genetics,physiology Conserved Sequence Corynebacterium/genetics,physiology Gene Expression Profiling Gene Expression Regulation, Bacterial Genes, Bacterial Heat-Shock Proteins/biosynthesis,genetics Heat-Shock Response/genetics Mycobacterium/genetics Operon Promoter Regions, Genetic Sigma Factor/genetics Streptomyces/genetics Transcription Initiation Site Transcription, Genetic
Chemicals
Bacterial Proteins Chaperonin 10 Chaperonin 60 Heat-Shock Proteins SigH protein, bacteria Sigma Factor
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Barreiro Carlos
Institute of Biotechnology of León (INBIOTEC). Science Park of León. Av. Real, 1, León, 24006, Spain.
González-Lavado Eva
Pátek Miroslav
Martín Juan-Francisco
References (25)
25 references, click to expand
  1. Role of the extracytoplasmic-function sigma factor sigma(H) in Mycobacterium tuberculosis global gene expression.
    Mol Microbiol. 2002 Jul;45(2):365-74 PMID: 12123450
  2. Defining the disulphide stress response in Streptomyces coelicolor A3(2): identification of the sigmaR regulon.
    Mol Microbiol. 2001 Nov;42(4):1007-20 PMID: 11737643
  3. Proteome analysis in the study of the bacterial heat-shock response.
    Mass Spectrom Rev. 2002 Jul-Aug;21(4):244-65 PMID: 12533799
  4. Comparative genomics reveal novel heat shock regulatory mechanisms in Staphylococcus aureus and other Gram-positive bacteria.
    Mol Microbiol. 2003 Feb;47(4):1061-73 PMID: 12581359
  5. Regulatory sequence analysis tools.
    Nucleic Acids Res. 2003 Jul 1;31(13):3593-6 PMID: 12824373
  6. Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens.
    Genome Res. 2003 Jul;13(7):1572-9 PMID: 12840036
  7. The Corynebacterium glutamicum genome: features and impacts on biotechnological processes.
    Appl Microbiol Biotechnol. 2003 Aug;62(2-3):99-109 PMID: 12743753
  8. Industrial production of amino acids by coryneform bacteria.
    J Biotechnol. 2003 Sep 4;104(1-3):155-72 PMID: 12948636
  9. Tools for genetic engineering in the amino acid-producing bacterium Corynebacterium glutamicum.
    J Biotechnol. 2003 Sep 4;104(1-3):287-99 PMID: 12948646
  10. Promoters of Corynebacterium glutamicum.
    J Biotechnol. 2003 Sep 4;104(1-3):311-23 PMID: 12948648
  11. Function of Corynebacterium glutamicum promoters in Escherichia coli, Streptomyces lividans, and Bacillus subtilis.
    J Biotechnol. 2003 Sep 4;104(1-3):325-34 PMID: 12948649
  12. Construction and characterization of promoter-probe vectors for Corynebacteria using the kanamycin-resistance reporter gene.
    Gene. 1991 Feb 1;98(1):117-21 PMID: 1849494
  13. CIRCE, a novel heat shock element involved in regulation of heat shock operon dnaK of Bacillus subtilis.
    J Bacteriol. 1994 Mar;176(5):1359-63 PMID: 8113175
  14. Identification of a Caulobacter crescentus operon encoding hrcA, involved in negatively regulating heat-inducible transcription, and the chaperone gene grpE.
    J Bacteriol. 1996 Apr;178(7):1829-41 PMID: 8606155
  15. Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif.
    Microbiology. 1996 May;142 ( Pt 5):1297-309 PMID: 8704969
  16. Disruption of hspR, the repressor gene of the dnaK operon in Streptomyces albus G.
    Mol Microbiol. 1997 Jan;23(1):77-84 PMID: 9004222
  17. The ClpB ATPase of Streptomyces albus G belongs to the HspR heat shock regulon.
    Mol Microbiol. 1999 Jan;31(2):521-32 PMID: 10027969
  18. Genome-wide transcription profiling of Corynebacterium glutamicum after heat shock and during growth on acetate and glucose.
    J Biotechnol. 2002 Sep 25;98(2-3):255-68 PMID: 12141991
  19. Heat shock protein 60 sequence comparisons: duplications, lateral transfer, and mitochondrial evolution.
    Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11348-53 PMID: 11027334
  20. Phylogenetic analysis of gram-positive bacteria based on grpE, encoded by the dnaK operon.
    Int J Syst Evol Microbiol. 2000 Sep;50 Pt 5:1761-6 PMID: 11034484
  21. The clpP multigenic family in Streptomyces lividans: conditional expression of the clpP3 clpP4 operon is controlled by PopR, a novel transcriptional activator.
    Mol Microbiol. 2000 Nov;38(3):602-12 PMID: 11069683
  22. Organization and transcriptional analysis of a six-gene cluster around the rplK-rplA operon of Corynebacterium glutamicum encoding the ribosomal proteins L11 and L1.
    Appl Environ Microbiol. 2001 May;67(5):2183-90 PMID: 11319098
  23. L-threonine export: use of peptides to identify a new translocator from Corynebacterium glutamicum.
    J Bacteriol. 2001 Sep;183(18):5317-24 PMID: 11514515
  24. The alternative sigma factor SigH regulates major components of oxidative and heat stress responses in Mycobacterium tuberculosis.
    J Bacteriol. 2001 Oct;183(20):6119-25 PMID: 11567012
  25. Negative regulation of the heat shock response in Streptomyces.
    Arch Microbiol. 2001 Oct;176(4):237-42 PMID: 11685367
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.186.14.4813-4817.2004
Published
2004-07-00
Pages
4813-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC438587
Subset
IM
Corrections
RetractionIn
PMID: 23661686
External Links
PubMed source DOI: 10.1128/JB.186.14.4813-4817.2004 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2