Analyzing cell cycle checkpoints after ionizing radiation.

Methods in molecular biology (Clifton, N.J.) ·Vol. 281 ·2004-00-00 ·Pages 283-92

Xu B, Kastan MB

Abstract

Several methods to measure cell cycle perturbation after exposure to ionizing radiation are presented in this chapter. These methods include the G1 checkpoint assay by 5' bromode-oxyuridine (BrdUrd) labeling followed by flow cytometric analysis, the S-phase checkpoint assay by measuring DNA synthesis in response to ionizing radiation, and the G2/M checkpoint assay by quantitating histone H3 phosphorylation after ionizing radiation.

MeSH Terms

Bromodeoxyuridine Cell Cycle/radiation effects DNA/metabolism Flow Cytometry/methods HeLa Cells Histones/metabolism Humans Phosphorylation/radiation effects Radiation, Ionizing Radiation-Sensitizing Agents

Chemicals

Histones Radiation-Sensitizing Agents DNA Bromodeoxyuridine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Xu Bo

Department of Genetics and Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, USA.

Kastan Michael B

Article Info

Journal

Methods in molecular biology (Clifton, N.J.)

Abbr.

Methods Mol Biol

ISSN

1064-3745

DOI

10.1385/1-59259-811-0:283

Published

2004-00-00

Pages

283-92

Language

English

Region

United States

NLM ID

9214969

Subset

External Links

PubMed source DOI: 10.1385/1-59259-811-0:283

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