Home LiteratureArticle Details
PMID: 15215149 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Enhancement of the enterocin CRL35 activity by a synthetic peptide derived from the NH2-terminal sequence.

Antimicrobial agents and chemotherapy ·Vol. 48 ·No. 7 ·2004-07-00 ·Pages 2778-81

Saavedra L, Minahk C, de Ruiz Holgado AP, Sesma F

Abstract

The enterocin CRL35 biosynthetic gene cluster was cloned and sequenced. The sequence was revealed to be highly identical to that of the mundticin KS gene cluster (S. Kawamoto, J. Shima, R. Sato, T. Eguchi, S. Ohmomo, J. Shibato, N. Horikoshi, K. Takeshita, and T. Sameshima, Appl. Environ. Microbiol. 68:3830-3840, 2002). Short synthetic peptides were designed based on the bacteriocin sequence and were evaluated in antimicrobial competitive assays. The peptide KYYGNGVSCNKKGCS produced an enhancement of enterocin CRL35 antimicrobial activity in a buffer system.

MeSH Terms
Amino Acid Sequence Bacteriocins/chemistry,genetics,pharmacology DNA Primers Drug Synergism Enterococcus/genetics Listeria/drug effects Membrane Potentials/drug effects Microbial Sensitivity Tests Molecular Sequence Data Peptides/chemical synthesis,pharmacology Reverse Transcriptase Polymerase Chain Reaction
Chemicals
Bacteriocins DNA Primers Peptides enterocin CRL35
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Saavedra Lucila
Centro de Referencia para Lactobacilos (CERELA-CONICET), S.M. de Tucumán (4000), Chacabuco 145, Tucumán, Argentina.
Minahk Carlos
de Ruiz Holgado Aída P
Sesma Fernando
References (19)
19 references, click to expand
  1. Preliminary characterization of bacteriocins produced by Enterococcus faecium and Enterococcus faecalis isolated from pig faeces.
    J Appl Microbiol. 2000 Mar;88(3):482-94 PMID: 10747229
  2. Antiviral activity of enterocin CRL35 against herpesviruses.
    Int J Antimicrob Agents. 1999 Aug;12(4):293-9 PMID: 10493605
  3. Bacteriocins: safe, natural antimicrobials for food preservation.
    Int J Food Microbiol. 2001 Dec 4;71(1):1-20 PMID: 11764886
  4. Ribosomally synthesized antibacterial peptides in Gram positive bacteria.
    Curr Drug Targets. 2002 Apr;3(2):107-22 PMID: 11958295
  5. Biochemical and genetic characterization of mundticin KS, an antilisterial peptide produced by Enterococcus mundtii NFRI 7393.
    Appl Environ Microbiol. 2002 Aug;68(8):3830-40 PMID: 12147478
  6. Enterocin CRL35 inhibits late stages of HSV-1 and HSV-2 replication in vitro.
    Antiviral Res. 2003 Mar;58(1):17-24 PMID: 12719003
  7. Inhibition of enterocin CRL35 antibiotic activity by mono- and divalent ions.
    Lett Appl Microbiol. 2003;37(5):374-9 PMID: 14633107
  8. Issues in pharmacokinetics and pharmacodynamics of anti-infective agents: kill curves versus MIC.
    Antimicrob Agents Chemother. 2004 Feb;48(2):369-77 PMID: 14742182
  9. Enhancement of antibiotic activity by sub-lethal concentrations of enterocin CRL35.
    J Antimicrob Chemother. 2004 Feb;53(2):240-6 PMID: 14729738
  10. Purification and N-terminal amino acid sequence of Enterocin CRL 35, a 'pediocin-like' bacteriocin produced by Enterococcus faecium CRL 35.
    Lett Appl Microbiol. 1996 Jun;22(6):417-9 PMID: 8695065
  11. Effect of amino acid substitutions on the activity of carnobacteriocin B2. Overproduction of the antimicrobial peptide, its engineered variants, and its precursor in Escherichia coli.
    J Biol Chem. 1997 Feb 7;272(6):3384-8 PMID: 9013580
  12. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
    Nucleic Acids Res. 1997 Sep 1;25(17):3389-402 PMID: 9254694
  13. Interactions of nisin and pediocin PA-1 with closely related lactic acid bacteria that manifest over 100-fold differences in bacteriocin sensitivity.
    Appl Environ Microbiol. 1997 Sep;63(9):3628-36 PMID: 9293015
  14. Electrostatic interactions, but not the YGNGV consensus motif, govern the binding of pediocin PA-1 and its fragments to phospholipid vesicles.
    Appl Environ Microbiol. 1997 Dec;63(12):4770-7 PMID: 9406395
  15. Lacticin 3147, a broad-spectrum bacteriocin which selectively dissipates the membrane potential.
    Appl Environ Microbiol. 1998 Feb;64(2):439-45 PMID: 9464377
  16. A novel bacteriocin with a YGNGV motif from vegetable-associated Enterococcus mundtii: full characterization and interaction with target organisms.
    Biochim Biophys Acta. 1998 Aug 14;1373(1):47-58 PMID: 9733915
  17. The bactericidal activity of pediocin PA-1 is specifically inhibited by a 15-mer fragment that spans the bacteriocin from the center toward the C terminus.
    Appl Environ Microbiol. 1998 Dec;64(12):5057-60 PMID: 9835610
  18. Lacticin 3147 displays activity in buffer against gram-positive bacterial pathogens which appear insensitive in standard plate assays.
    Lett Appl Microbiol. 1999 May;28(5):355-8 PMID: 10347889
  19. Analogues of bacteriocins: antimicrobial specificity and interactions of leucocin A with its enantiomer, carnobacteriocin B2, and truncated derivatives.
    J Med Chem. 2000 Nov 30;43(24):4579-81 PMID: 11101349
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.48.7.2778-2781.2004
Published
2004-07-00
Pages
2778-81
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC434193
Subset
IM
Grants
PHS HHS · AY 398693 · United States
External Links
PubMed source DOI: 10.1128/AAC.48.7.2778-2781.2004 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2