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PMID: 15173221 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Recoverin regulates light-dependent phosphodiesterase activity in retinal rods.

The Journal of general physiology ·Vol. 123 ·No. 6 ·2004-06-00 ·Pages 729-41

Makino CL, Dodd RL, Chen J, Burns ME, Roca A, Simon MI, Baylor DA

Abstract

The Ca2+-binding protein recoverin may regulate visual transduction in retinal rods and cones, but its functional role and mechanism of action remain controversial. We compared the photoresponses of rods from control mice and from mice in which the recoverin gene was knocked out. Our analysis indicates that Ca2+-recoverin prolongs the dark-adapted flash response and increases the rod's sensitivity to dim steady light. Knockout rods had faster Ca2+ dynamics, indicating that recoverin is a significant Ca2+ buffer in the outer segment, but incorporation of exogenous buffer did not restore wild-type behavior. We infer that Ca2+-recoverin potentiates light-triggered phosphodiesterase activity, probably by effectively prolonging the catalytic activity of photoexcited rhodopsin.

MeSH Terms
Adaptation, Physiological/physiology,radiation effects Animals Calcium Signaling/physiology,radiation effects Calcium-Binding Proteins/deficiency,genetics,metabolism Cells, Cultured Dose-Response Relationship, Radiation Enzyme Activation/radiation effects Eye Proteins/genetics,metabolism Light Lipoproteins/deficiency,genetics,metabolism Membrane Potentials/drug effects,physiology Mice Mice, Inbred C57BL Mice, Knockout Phosphoric Diester Hydrolases/metabolism Recombinant Proteins/metabolism Recoverin Retinal Rod Photoreceptor Cells/physiology,radiation effects
Chemicals
Calcium-Binding Proteins Eye Proteins Lipoproteins Rcvrn protein, mouse Recombinant Proteins Recoverin Phosphoric Diester Hydrolases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Makino Clint L
Department of Ophthalmology, Harvard Medical School and the Massachusetts Eye and Ear Infirmary, 243 Charles Street, Boston, MA 02114, USA. cmakino@meei.harvard.edu
Dodd R L
Chen J
Burns M E
Roca A
Simon M I
Baylor D A
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.200308994
Email
cmakino@meei.harvard.edu
Published
2004-06-00
Pages
729-41
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2234569
Subset
IM
Grants
NEI NIH HHS · R01 EY014047-04 · United States
NEI NIH HHS · R01 EY014047 · United States
NEI NIH HHS · R01 EY012703 · United States
NEI NIH HHS · EY12703 · United States
NEI NIH HHS · EY05750 · United States
NEI NIH HHS · R01 EY012944 · United States
NEI NIH HHS · EY14047 · United States
NIA NIH HHS · AG12288 · United States
NEI NIH HHS · EY12944 · United States
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1085/jgp.200308994 PMC Full Text View journal details
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