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PMID: 15170475 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

The J-protein family: modulating protein assembly, disassembly and translocation.

EMBO reports ·Vol. 5 ·No. 6 ·2004-06-00 ·Pages 567-71

Walsh P, Bursać D, Law YC, Cyr D, Lithgow T

Abstract

DnaJ is a molecular chaperone and the prototypical member of the J-protein family. J proteins are defined by the presence of a J domain that can regulate the activity of 70-kDa heat-shock proteins. Sequence analysis on the genome of Saccharomyces cerevisiae has revealed 22 proteins that establish four distinguishing structural features of the J domain: predicted helicity in segments I-IV, precisely placed interhelical contact residues, a lysine-rich surface on helix II and placement of the diagnostic sequence HPD between the predicted helices II and III. We suggest that this definition of the J-protein family could be used for other genome-wide studies. In addition, three J-like proteins were identified in yeast that contain regions closely resembling a J domain, but in which the HPD motif is non-conservatively replaced. We suggest that J-like proteins might function to regulate the activity of bona fide J proteins during protein translocation, assembly and disassembly.

MeSH Terms
Amino Acid Sequence Genome, Fungal Heat-Shock Proteins/classification,physiology Molecular Chaperones/classification,physiology Molecular Sequence Data Protein Structure, Tertiary Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/classification,physiology Sequence Alignment
Chemicals
Heat-Shock Proteins Molecular Chaperones Saccharomyces cerevisiae Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Walsh Peter
Russell Grimwade School of Biochemistry and Molecular Biology, University of Melbourne, Melbourne 3010, Australia.
Bursać Dejan
Law Yin Chern
Cyr Douglas
Lithgow Trevor
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Article Info
Journal
EMBO reports
Abbr.
EMBO Rep
ISSN
1469-221X
DOI
10.1038/sj.embor.7400172
Published
2004-06-00
Pages
567-71
Language
English
Region
England
NLM ID
100963049
PMCID
PMC1299080
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.embor.7400172 PMC Full Text View journal details
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