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PMID: 15169917 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dynamics of protein binding to telomeres in living cells: implications for telomere structure and function.

Molecular and cellular biology ·Vol. 24 ·No. 12 ·2004-06-00 ·Pages 5587-94

Mattern KA, Swiggers SJ, Nigg AL, Löwenberg B, Houtsmuller AB, Zijlmans JM

Abstract

Telomeric proteins have an essential role in the regulation of the length of the telomeric DNA tract and in protection against end-to-end chromosome fusion. Telomere organization and how individual proteins are involved in different telomere functions in living cells is largely unknown. By using green fluorescent protein tagging and photobleaching, we investigated in vivo interactions of human telomeric DNA-binding proteins with telomeric DNA. Our results show that telomeric proteins interact with telomeres in a complex dynamic fashion: TRF2, which has a dual role in chromosome end protection and telomere length homeostasis, resides at telomeres in two distinct pools. One fraction ( approximately 73%) has binding dynamics similar to TRF1 (residence time of approximately 44 s). Interestingly, the other fraction of TRF2 binds with similar dynamics as the putative end-protecting factor hPOT1 (residence time of approximately 11 min). Our data support a dynamic model of telomeres in which chromosome end-protection and telomere length homeostasis are governed by differential binding of telomeric proteins to telomeric DNA.

MeSH Terms
Green Fluorescent Proteins HeLa Cells Humans Kinetics Luminescent Proteins/genetics,metabolism Protein Binding Recombinant Fusion Proteins/genetics,metabolism Shelterin Complex Telomere/genetics,metabolism Telomere-Binding Proteins/genetics,metabolism Telomeric Repeat Binding Protein 1/genetics,metabolism Telomeric Repeat Binding Protein 2/genetics,metabolism
Chemicals
Luminescent Proteins POT1 protein, human Recombinant Fusion Proteins Shelterin Complex Telomere-Binding Proteins Telomeric Repeat Binding Protein 1 Telomeric Repeat Binding Protein 2 Green Fluorescent Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mattern Karin A
Department of Hematology, Erasmus MC, 3000DR Rotterdam, The Netherlands.
Swiggers Susan J J
Nigg Alex L
Löwenberg Bob
Houtsmuller Adriaan B
Zijlmans J Mark J M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.24.12.5587-5594.2004
Published
2004-06-00
Pages
5587-94
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC419875
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.24.12.5587-5594.2004 PMC Full Text View journal details
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