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PMID: 15157893 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of chromatin in fission yeast.

Methods (San Diego, Calif.) ·Vol. 33 ·No. 3 ·2004-07-00 ·Pages 252-9

Pidoux A, Mellone B, Allshire R

Abstract

The use of fission yeast as a model system for studies of chromosome biology has contributed to several key advances in the last few years. The structure of its large complex centromeres and composition of its transcriptionally silent heterochromatin resemble those of metazoa. The application of chromatin immunoprecipitation to fission yeast has been instrumental in these advances and we describe an improved version of this technique in detail. In addition, we describe several other techniques, which are useful in the analysis of chromatin in fission yeast.

MeSH Terms

Chromatin/chemistry,metabolism Schizosaccharomyces/chemistry,metabolism Schizosaccharomyces pombe Proteins/analysis,metabolism

Chemicals

Chromatin Schizosaccharomyces pombe Proteins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Pidoux Alison

Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, Swann Building, King's Buildings, University of Edinburgh, UK. Alison.Pidoux@ed.ac.uk

Mellone Barbara

Allshire Robin

Article Info

Journal

Methods (San Diego, Calif.)

Abbr.

Methods

ISSN

1046-2023

DOI

10.1016/j.ymeth.2003.11.021

alison.pidoux@ed.ac.uk

Published

2004-07-00

Pages

252-9

Language

English

Region

United States

NLM ID

9426302

Subset

External Links

PubMed source DOI: 10.1016/j.ymeth.2003.11.021 View journal details

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Analysis of chromatin in fission yeast.

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