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PMID: 151534 Published · ppublish English Journal Article

Characterization of a region of the primary sequence of troponin C involved in calcium ion-dependent interaction with troponin I.

The Biochemical journal ·Vol. 173 ·No. 2 ·1978-08-01 ·Pages 449-57

Weeks RA, Perry SV

Abstract

1. The CNBr digest of troponin C from rabbit fast skeletal muscle was shown to possess many of the functional properties of the whole troponin C molecule. 2. A peptide corresponding to residues 83-134 was isolated, which forms a Ca(2+-dependent complex with troponin I and neutralizes the inhibition by troponin I of the Mg(2+-stimulated adenosine triphosphatase of desensitized actomyosin. 3. The peptide inhibits the phosphorylation of fast-skeletal-muscle, but not cardiac-muscle, troponin I, by 3' :5'-cyclic AMP-dependent protein kinase. In this property it was as effective as whole skeletal-muscle troponin C when compared on a molar basis. 4. Biological activity was also present in other fractions obtained from the CNBr digest. 5. By gel filtration and affinity chromatography of the whole CNBr digest of troponin C, two peptides, one of which was identified as representing residues 83-134, were shown to form Ca(2+-dependent complexes with troponin I. 6. The significance of these findings for the mechanism of interaction of troponin C and troponin I is discussed.

MeSH Terms
Adenosine Triphosphatases/antagonists & inhibitors Amino Acids/analysis Calcium/metabolism Chromatography, Affinity Chromatography, Gel Cyanogen Bromide Electrophoresis, Polyacrylamide Gel Muscle Proteins/metabolism Peptide Fragments/isolation & purification,pharmacology Protein Kinase Inhibitors Troponin/metabolism
Chemicals
Amino Acids Muscle Proteins Peptide Fragments Protein Kinase Inhibitors Troponin Adenosine Triphosphatases Cyanogen Bromide Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Weeks R A
Perry S V
References (29)
29 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1730449
Published
1978-08-01
Pages
449-57
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1185798
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1730449 PMC Full Text View journal details
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