Functional expression of the ectoine hydroxylase gene (thpD) from Streptomyces chrysomallus in Halomonas elongata.

Applied and environmental microbiology ·Vol. 70 ·No. 5 ·2004-05-00 ·Pages 3130-2

Prabhu J, Schauwecker F, Grammel N, Keller U, Bernhard M

Abstract

The formation of hydroxyectoine in the industrial ectoine producer Halomonas elongata was improved by the heterologous expression of the ectoine hydroxylase gene, thpD, from Streptomyces chrysomallus. The efficient conversion of ectoine to hydroxyectoine was achieved by the concerted regulation of thpD by the H. elongata ectA promoter.

MeSH Terms

Amino Acids, Diamino/biosynthesis,metabolism Bacterial Proteins/genetics,metabolism Biotechnology/methods Gene Expression Regulation, Bacterial Halomonas/enzymology,genetics Mixed Function Oxygenases/genetics,metabolism Molecular Sequence Data Sequence Analysis, DNA Streptomyces/enzymology,genetics

Chemicals

Amino Acids, Diamino Bacterial Proteins hydroxyectoine ectoine Mixed Function Oxygenases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Prabhu Julia

ActinoDrug Pharmaceuticals GmbH, 16761 Hennigsdorf, Germany.

Schauwecker Florian

Grammel Nicolas

Keller Ullrich

Bernhard Michael

References (13)

13 references, click to expand

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/AEM.70.5.3130-3132.2004

Published

2004-05-00

Pages

3130-2

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC404422

Subset

Databases

GENBANK

AY524544

External Links

PubMed source DOI: 10.1128/AEM.70.5.3130-3132.2004 PMC Full Text View journal details

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Functional expression of the ectoine hydroxylase gene (thpD) from Streptomyces chrysomallus in Halomonas elongata.

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