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PMID: 1511881 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of genetic expression and secretion signals from Enterococcus faecalis through the use of broad-host-range alpha-amylase probe vectors.

Gene ·Vol. 118 ·No. 1 ·1992-09-01 ·Pages 21-30

Hols P, Baulard A, Garmyn D, Delplace B, Hogan S, Delcour J

Abstract

We have constructed two broad-host-range Gram+/Gram- probe vectors designed for the cloning of bacterial genetic expression and secretion signals. These vectors make use of a silent reporter gene encoding the mature alpha-amylase from Bacillus licheniformis whose reactivation can easily be monitored on iodine-stained starch plates. Shotgun cloning of Enterococcus faecalis DNA fragments allowed recovery of several cassettes directing transcription, translation of the reporter gene and secretion of alpha-amylase. Sequence analysis revealed, in each case, the presence of a putative promoter, ribosome-binding site and signal peptide similar to those described in other Gram+ bacteria.

Related Genes

amyL

MeSH Terms

Amino Acid Sequence Bacillus/enzymology,genetics Base Sequence Chromosomes, Bacterial Cloning, Molecular/methods Enterococcus faecalis/genetics Escherichia coli/genetics Gene Expression Regulation, Bacterial Genetic Vectors/genetics Molecular Sequence Data Mutagenesis, Insertional/genetics Protein Biosynthesis Protein Conformation Protein Sorting Signals/genetics Recombinant Fusion Proteins/genetics Restriction Mapping Sequence Homology, Nucleic Acid Transcription, Genetic alpha-Amylases/genetics,metabolism

Chemicals

Protein Sorting Signals Recombinant Fusion Proteins alpha-Amylases

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Hols P

Unité de Génétique, Université Catholique de Louvain, Louvain-La-Neuve, Belgium.

Baulard A

Garmyn D

Delplace B

Hogan S

Delcour J

Article Info

Journal

Gene

Abbr.

Gene

ISSN

0378-1119

DOI

10.1016/0378-1119(92)90244-j

Published

1992-09-01

Pages

21-30

Language

English

Region

Netherlands

NLM ID

7706761

Subset

Databases

GENBANK

M74891, M74892, M77275, M77276, M77277, M77278, M77279, M79309, M79310, M90357, S43285, S43286, S43293, S43295, S43297

External Links

PubMed source DOI: 10.1016/0378-1119(92)90244-j View journal details

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Isolation and characterization of genetic expression and secretion signals from Enterococcus faecalis through the use of broad-host-range alpha-amylase probe vectors.

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