Home LiteratureArticle Details
PMID: 1511125 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A 16 kb small single-copy region separates the plastid DNA inverted repeat of the unicellular red alga Cyanidium caldarium: physical mapping of the IR-flanking regions and nucleotide sequences of the psbD-psbC, rps16, 5S rRNA and rpl21 genes.

Plant molecular biology ·Vol. 19 ·No. 6 ·1992-09-00 ·Pages 1001-10

Maid U, Zetsche K

Abstract

The four inverted repeat (IR) flanking regions of the Cyanidium caldarium plastid DNA were cloned. Southern blotting, transcript and sequence analyses of the border regions revealed the psbD-psbC operon and the rps16 gene within the large single-copy region upstream of the 16S rDNA gene and the rpl21 gene downstream of the 5S rDNA within the 16 kb small single-copy region. The size of the IR is ca. 5 kb. The nucleotide sequences of the psbD-psbC, rps16, rpl21 and 5S rRNA genes with the corresponding alignments and physical maps of the regions are presented. Northern analysis revealed a less complex psbD-psbC transcription pattern than has been found in higher plants. Comparisons to other red algal data point to structural diversity within red algal plastid DNA.

Related Genes
psbC psbD rpl21 rps16
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Chloroplasts/metabolism DNA/metabolism Molecular Sequence Data Photosynthetic Reaction Center Complex Proteins/genetics RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 5S/genetics Repetitive Sequences, Nucleic Acid Restriction Mapping Rhodophyta/genetics Sequence Homology, Nucleic Acid
Chemicals
Photosynthetic Reaction Center Complex Proteins RNA, Ribosomal, 16S RNA, Ribosomal, 5S DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Maid U
Institut für Pflanzenphysiologie, Justus Liebig Universität, Giessen, Germany.
Zetsche K
References (30)
30 references, click to expand
  1. Studies with Cyanidium caldarium, an anomalously pigmented chlorophyte.
    Arch Mikrobiol. 1959;32(3):270-7 PMID: 13628094
  2. Chloroplast mRNA 3' end processing requires a nuclear-encoded RNA-binding protein.
    EMBO J. 1991 Jun;10(6):1493-502 PMID: 2026146
  3. Peculiar feature of the organization of rRNA genes of the Chlorella chloroplast DNA.
    Nucleic Acids Res. 1986 May 12;14(9):3827-39 PMID: 3714498
  4. Chloroplast genomes of two conifers lack a large inverted repeat and are extensively rearranged.
    Proc Natl Acad Sci U S A. 1988 Jun;85(11):3898-902 PMID: 2836862
  5. Nucleotide sequence of the plastid 16S rRNA gene of the red alga Cyanidium caldarium.
    Nucleic Acids Res. 1990 Jul 11;18(13):3996 PMID: 2374722
  6. Sequence of the chloroplast 16S rRNA gene and its surrounding regions of Chlamydomonas reinhardii.
    Nucleic Acids Res. 1982 Dec 11;10(23):7609-20 PMID: 6296784
  7. Light-dependent changes in psbD and psbC transcripts of barley chloroplasts: accumulation of two transcripts maintains psbD and psbC translation capability in mature chloroplasts.
    EMBO J. 1988 May;7(5):1289-97 PMID: 3044778
  8. Hypothesis for the evolutionary origin of the chloroplast ribosomal protein L21 of spinach.
    Curr Genet. 1990 Dec;18(6):553-6 PMID: 2076556
  9. Nucleotide sequence of rye chloroplast DNA fragment, comprising psbD, psbC and trnS genes.
    Nucleic Acids Res. 1989 Jan 25;17(2):798 PMID: 2644624
  10. Organization and nucleotide sequence of ribosomal RNA genes on a circular 73 kbp DNA from the colourless flagellate Astasia longa.
    Curr Genet. 1990 May;17(5):433-8 PMID: 2113436
  11. Plastid DNA from Pyrenomonas salina (Cryptophyceae): physical map, genes, and evolutionary implications.
    Curr Genet. 1992 Jan;21(1):73-81 PMID: 1735127
  12. Structure of the rubisco operon from the multicellular red alga Antithamnion spec.
    Curr Genet. 1990 Dec;18(5):465-9 PMID: 2078870
  13. Structure and expression of a plastid-encoded groEL homologous heat-shock gene in a thermophilic unicellular red alga.
    Curr Genet. 1992 May;21(6):521-5 PMID: 1352188
  14. Compilation of 5S rRNA and 5S rRNA gene sequences.
    Nucleic Acids Res. 1990 Apr 25;18 Suppl:2215-30 PMID: 1692116
  15. Structural similarities between psbA genes from red and brown algae.
    Curr Genet. 1991 Jul;20(1-2):177-80 PMID: 1934114
  16. CLUSTAL: a package for performing multiple sequence alignment on a microcomputer.
    Gene. 1988 Dec 15;73(1):237-44 PMID: 3243435
  17. The 5S ribosomal RNA sequences of a red algal rhodoplast and a gymnosperm chloroplast. Implications for the evolution of plastids and cyanobacteria.
    J Mol Evol. 1988;27(2):126-32 PMID: 3137350
  18. Rubisco genes indicate a close phylogenetic relation between the plastids of Chromophyta and Rhodophyta.
    Plant Mol Biol. 1990 Oct;15(4):575-84 PMID: 2102375
  19. The genes of both subunits of ribulose-1,5-bisphosphate carboxylase constitute an operon on the plastome of a red alga.
    Curr Genet. 1989 Sep;16(3):203-9 PMID: 2598276
  20. Comparative organization of chloroplast genomes.
    Annu Rev Genet. 1985;19:325-54 PMID: 3936406
  21. Chloroplast genome characterization in the red alga Griffithsia pacifica.
    Mol Gen Genet. 1987 Sep;209(2):343-51 PMID: 17191345
  22. Structure of the Rubisco operon from the unicellular red alga Cyanidium caldarium: evidence for a polyphyletic origin of the plastids.
    Mol Gen Genet. 1990 Jul;222(2-3):425-30 PMID: 2274041
  23. Repetitive sequence-mediated rearrangements in Chlorella ellipsoidea chloroplast DNA: completion of nucleotide sequence of the large inverted repeat.
    Curr Genet. 1991 Feb;19(2):139-47 PMID: 2065363
  24. Junctions of the large single copy region and the inverted repeats in Spinacia oleracea and Nicotiana debneyi chloroplast DNA: sequence of the genes for tRNAHis and the ribosomal proteins S19 and L2.
    Nucleic Acids Res. 1984 Aug 24;12(16):6547-58 PMID: 6089120
  25. Structural features of the plastid ribosomal RNA operons of two red algae: Antithamnion sp. and Cyanidium caldarium.
    Plant Mol Biol. 1991 Apr;16(4):537-46 PMID: 1868197
  26. Unusual characteristics of Codium fragile chloroplast DNA revealed by physical and gene mapping.
    Mol Gen Genet. 1989 Apr;216(2-3):417-21 PMID: 2747622
  27. An additional promoter within the protein-coding region of the psbD-psbC gene cluster in tobacco chloroplast DNA.
    Nucleic Acids Res. 1989 Dec 11;17(23):9583-91 PMID: 2481263
  28. The psbA-gene from a red alga resembles those from cyanobacteria and cyanelles.
    Curr Genet. 1990 Mar;17(3):255-9 PMID: 2111229
  29. Extension of a flexible computer program for handling DNA sequence data.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):113-20 PMID: 6694895
  30. An equivalent to bacterial ompR genes is encoded on the plastid genome of red algae.
    Plant Mol Biol. 1992 Feb;18(4):777-80 PMID: 1558950
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00040531
Published
1992-09-00
Pages
1001-10
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
X62578
External Links
PubMed source DOI: 10.1007/BF00040531 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2