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PMID: 15064376 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tissue-specific localization of an abscisic acid biosynthetic enzyme, AAO3, in Arabidopsis.

Plant physiology ·Vol. 134 ·No. 4 ·2004-04-00 ·Pages 1697-707

Koiwai H, Nakaminami K, Seo M, Mitsuhashi W, Toyomasu T, Koshiba T

Abstract

Arabidopsis aldehyde oxidase 3 (AAO3) is an enzyme involved in abscisic acid (ABA) biosynthesis in response to drought stress. Since the enzyme catalyzes the last step of the pathway, ABA production sites may be determined by the presence of AAO3. Here, AAO3 localization was investigated using AAO3 promoter:AAO3-GFP transgenic plants and by an immunohistochemical technique. AAO3-GFP protein exhibited an activity to produce ABA from abscisic aldehyde, and the transgene restored the wilty phenotype of the aao3 mutant. GFP-fluorescence was detected in the root tips, vascular bundles of roots, hypocotyls and inflorescence stems, and along the leaf veins. Intense immunofluorescence signals were localized in phloem companion cells and xylem parenchyma cells. Faint but significant GFP- and immuno-fluorescence signals were observed in the leaf guard cells. In situ hybridization with antisense AAO3 mRNA showed AAO3 mRNA expression in the guard cells of dehydrated leaves. These results indicate that the ABA synthesized in vascular systems is transported to various target tissues and cells, and also that the guard cells themselves are able to synthesize ABA.

MeSH Terms
Abscisic Acid/biosynthesis Aldehyde Oxidase/genetics,metabolism Arabidopsis/enzymology,genetics,metabolism Arabidopsis Proteins/genetics,metabolism Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Green Fluorescent Proteins Immunohistochemistry Luminescent Proteins/genetics,metabolism Molecular Sequence Data Plant Epidermis/enzymology,genetics,metabolism Plants, Genetically Modified RNA, Messenger/genetics,metabolism Recombinant Fusion Proteins/genetics,metabolism Signal Transduction/genetics,physiology Substrate Specificity
Chemicals
Arabidopsis Proteins Luminescent Proteins RNA, Messenger Recombinant Fusion Proteins Green Fluorescent Proteins Abscisic Acid AAO3 protein, Arabidopsis Aldehyde Oxidase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Koiwai Hanae
Department of Biological Sciences, Tokyo Metropolitan University, Hachioji-shi, Tokyo 192-0397, Japan.
Nakaminami Kentaro
Seo Mitsunori
Mitsuhashi Wataru
Toyomasu Tomonobu
Koshiba Tomokazu
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.103.036970
Published
2004-04-00
Epub
2004-00-02
Pages
1697-707
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC419843
Subset
IM
Databases
GENBANK
AB016622
External Links
PubMed source DOI: 10.1104/pp.103.036970 PMC Full Text View journal details
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