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PMID: 15060174 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27.

Molecular and cellular biology ·Vol. 24 ·No. 8 ·2004-04-00 ·Pages 3562-76

Schwickart M, Havlis J, Habermann B, Bogdanova A, Camasses A, Oelschlaegel T, Shevchenko A, Zachariae W

Abstract

The anaphase-promoting complex (APC/C) is a large ubiquitin-protein ligase which controls progression through anaphase by triggering the degradation of cell cycle regulators such as securin and B-type cyclins. The APC/C is an unusually complex ligase containing at least 10 different, evolutionarily conserved components. In contrast to APC/C's role in cell cycle regulation little is known about the functions of individual subunits and how they might interact with each other. Here, we have analyzed Swm1/Apc13, a small subunit recently identified in the budding yeast complex. Database searches revealed proteins related to Swm1/Apc13 in various organisms including humans. Both the human and the fission yeast homologues are associated with APC/C subunits, and they complement the phenotype of an SWM1 deletion mutant of budding yeast. Swm1/Apc13 promotes the stable association with the APC/C of the essential subunits Cdc16 and Cdc27. Accordingly, Swm1/Apc13 is required for ubiquitin ligase activity in vitro and for the timely execution of APC/C-dependent cell cycle events in vivo.

MeSH Terms
Amino Acid Sequence Anaphase-Promoting Complex-Cyclosome Animals Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome Cell Cycle/physiology Cell Cycle Proteins/metabolism Chromatids/metabolism DNA Polymerase III Evolution, Molecular Genetic Complementation Test Humans Meiosis/physiology Molecular Sequence Data Protein Subunits/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Schizosaccharomyces/genetics,metabolism Schizosaccharomyces pombe Proteins Sequence Alignment Ubiquitin-Protein Ligase Complexes/chemistry,genetics,metabolism Ubiquitin-Protein Ligases
Chemicals
Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome CDC27 protein, S cerevisiae CDC27 protein, human Cell Cycle Proteins Protein Subunits Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins cdc27 protein, S pombe Ubiquitin-Protein Ligase Complexes Anaphase-Promoting Complex-Cyclosome Ubiquitin-Protein Ligases DNA Polymerase III SWM1 protein, S cerevisiae
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Schwickart Martin
Max Planck Institute of Molecular Cell Biology and Genetics. Scionics Computer Innovation GmbH, Dresden, Germany.
Havlis Jan
Habermann Bianca
Bogdanova Aliona
Camasses Alain
Oelschlaegel Tobias
Shevchenko Andrej
Zachariae Wolfgang
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.24.8.3562-3576.2004
Published
2004-04-00
Pages
3562-76
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC381669
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.24.8.3562-3576.2004 PMC Full Text View journal details
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