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PMID: 150601 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Induction of ouabain-resistant mutations in C3H 10T1/2 mouse cells by ultraviolet light.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 7 ·1978-07-00 ·Pages 3363-6

Chan GL, Little JB

Abstract

Ouabain-resistant mutants were induced in C3H mouse embryo 10T1/2 fibroblasts by exposure to ultraviolet light, thus making available an in vitro system for studying mutagenesis and oncogenic transformation in parallel. 86Rb uptake studies showed that biochemical mutants at the plasma membrane Na+,K+ transport ATPase (EC 3.6.1.3) locus were being selected for in this system. The optimal expression time for the mutants was found to depend on the dose of ultraviolet light, as was the induced mutation frequency. The ratio of transformation to mutation frequencies was found to be on the order of 10 for four different doses, suggesting that the target size in the cellular genome for transformation may be approximately 10 times the size of the Na+,K+ ATPase gene. We propose that both transformation and mutation induction can now be quantitatively studied in this single system.

MeSH Terms
Adenosine Triphosphatases/metabolism Animals Biological Transport, Active/drug effects Cell Line Cell Survival/drug effects Cell Transformation, Neoplastic Dose-Response Relationship, Radiation Drug Resistance Mice Mice, Inbred C3H Mutation/radiation effects Ouabain/pharmacology Rubidium/metabolism Ultraviolet Rays
Chemicals
Ouabain Adenosine Triphosphatases Rubidium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chan G L
Little J B
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.7.3363
Published
1978-07-00
Pages
3363-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392776
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.7.3363 PMC Full Text View journal details
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