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PMID: 15060053 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Protein-protein interactions of proteins from the ESAT-6 family of Mycobacterium tuberculosis.

Journal of bacteriology ·Vol. 186 ·No. 8 ·2004-04-00 ·Pages 2487-91

Okkels LM, Andersen P

Abstract

In the present study, we demonstrate that, in analogy with the genes encoding ESAT-6 and CFP-10, the genes rv0287 and rv0288 from the ESAT-6 gene family are cotranscribed. Using Western-Western blotting and protein-print overlay methodologies, we demonstrate that ESAT-6 and CFP-10, as well as the protein pair Rv0288/Rv0287, interact pairwise in a highly specific way. Most notably, the ESAT-6 proteins interact directly with Rv3873, a possible cell envelope component of the ESAT-6 secretion pathway.

MeSH Terms
Antigens, Bacterial/genetics,metabolism Bacterial Proteins/genetics,metabolism Mycobacterium tuberculosis/genetics,metabolism Protein Binding Sequence Homology, Amino Acid Species Specificity
Chemicals
Antigens, Bacterial Bacterial Proteins CFP 6 protein, Mycobacterium tuberculosis CFP-10 protein, Mycobacterium tuberculosis
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Okkels Limei Meng
Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, DK-2300, Denmark. okkels@mpiib-berlin.mpg.de
Andersen Peter
References (29)
29 references, click to expand
  1. An esat6 knockout mutant of Mycobacterium bovis produced by homologous recombination will contribute to the development of a live tuberculosis vaccine.
    Tuber Lung Dis. 2000;80(4-5):185-9 PMID: 11052907
  2. The mycosins of Mycobacterium tuberculosis H37Rv: a family of subtilisin-like serine proteases.
    Gene. 2000 Aug 22;254(1-2):147-55 PMID: 10974545
  3. Massive gene decay in the leprosy bacillus.
    Nature. 2001 Feb 22;409(6823):1007-11 PMID: 11234002
  4. Comparing genomes within the species Mycobacterium tuberculosis.
    Genome Res. 2001 Apr;11(4):547-54 PMID: 11282970
  5. The Mycobacterium tuberculosis ECF sigma factor sigmaE: role in global gene expression and survival in macrophages.
    Mol Microbiol. 2001 Jul;41(2):423-37 PMID: 11489128
  6. The ESAT-6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram-positive bacteria.
    Genome Biol. 2001;2(10):RESEARCH0044 PMID: 11597336
  7. Tuberculin skin testing compared with T-cell responses to Mycobacterium tuberculosis-specific and nonspecific antigens for detection of latent infection in persons with recent tuberculosis contact.
    Clin Diagn Lab Immunol. 2001 Nov;8(6):1089-96 PMID: 11687445
  8. The ESAT-6/WXG100 superfamily -- and a new Gram-positive secretion system?
    Trends Microbiol. 2002 May;10(5):209-12 PMID: 11973144
  9. Conclusive evidence that the major T-cell antigens of the Mycobacterium tuberculosis complex ESAT-6 and CFP-10 form a tight, 1:1 complex and characterization of the structural properties of ESAT-6, CFP-10, and the ESAT-6*CFP-10 complex. Implications for pathogenesis and virulence.
    J Biol Chem. 2002 Jun 14;277(24):21598-603 PMID: 11940590
  10. Epitope mapping of the immunodominant antigen TB10.4 and the two homologous proteins TB10.3 and TB12.9, which constitute a subfamily of the esat-6 gene family.
    Infect Immun. 2002 Oct;70(10):5446-53 PMID: 12228269
  11. Loss of RD1 contributed to the attenuation of the live tuberculosis vaccines Mycobacterium bovis BCG and Mycobacterium microti.
    Mol Microbiol. 2002 Nov;46(3):709-17 PMID: 12410828
  12. Deletion of RD1 from Mycobacterium tuberculosis mimics bacille Calmette-Guérin attenuation.
    J Infect Dis. 2003 Jan 1;187(1):117-23 PMID: 12508154
  13. Mycosin-1, a subtilisin-like serine protease of Mycobacterium tuberculosis, is cell wall-associated and expressed during infection of macrophages.
    BMC Microbiol. 2002 Oct 7;2:30 PMID: 12366866
  14. Genes required for mycobacterial growth defined by high density mutagenesis.
    Mol Microbiol. 2003 Apr;48(1):77-84 PMID: 12657046
  15. Recombinant BCG exporting ESAT-6 confers enhanced protection against tuberculosis.
    Nat Med. 2003 May;9(5):533-9 PMID: 12692540
  16. Genomic approach to identification of Mycobacterium bovis diagnostic antigens in cattle.
    J Clin Microbiol. 2003 Aug;41(8):3719-28 PMID: 12904381
  17. PPE protein (Rv3873) from DNA segment RD1 of Mycobacterium tuberculosis: strong recognition of both specific T-cell epitopes and epitopes conserved within the PPE family.
    Infect Immun. 2003 Nov;71(11):6116-23 PMID: 14573626
  18. Properties of proteins MPB64, MPB70, and MPB80 of Mycobacterium bovis BCG.
    Infect Immun. 1986 Apr;52(1):293-302 PMID: 3514457
  19. Characterization of the major membrane protein of virulent Mycobacterium tuberculosis.
    Infect Immun. 1992 May;60(5):2066-74 PMID: 1563797
  20. Recall of long-lived immunity to Mycobacterium tuberculosis infection in mice.
    J Immunol. 1995 Apr 1;154(7):3359-72 PMID: 7897219
  21. Purification and characterization of a low-molecular-mass T-cell antigen secreted by Mycobacterium tuberculosis.
    Infect Immun. 1995 May;63(5):1710-7 PMID: 7729876
  22. B-cell epitopes and quantification of the ESAT-6 protein of Mycobacterium tuberculosis.
    Infect Immun. 1998 Feb;66(2):717-23 PMID: 9453632
  23. A Mycobacterium tuberculosis operon encoding ESAT-6 and a novel low-molecular-mass culture filtrate protein (CFP-10).
    Microbiology. 1998 Nov;144 ( Pt 11):3195-203 PMID: 9846755
  24. Differential protective efficacy of DNA vaccines expressing secreted proteins of Mycobacterium tuberculosis.
    Infect Immun. 1999 Apr;67(4):1702-7 PMID: 10085007
  25. Search for genes potentially involved in Mycobacterium tuberculosis virulence by mRNA differential display.
    Biochem Biophys Res Commun. 1999 Apr 29;258(1):94-101 PMID: 10222241
  26. Comparative evaluation of low-molecular-mass proteins from Mycobacterium tuberculosis identifies members of the ESAT-6 family as immunodominant T-cell antigens.
    Infect Immun. 2000 Jan;68(1):214-20 PMID: 10603390
  27. ESAT-6 subunit vaccination against Mycobacterium tuberculosis.
    Infect Immun. 2000 Feb;68(2):791-5 PMID: 10639447
  28. Analysis of the proteome of Mycobacterium tuberculosis in silico.
    Tuber Lung Dis. 1999;79(6):329-42 PMID: 10694977
  29. Towards the proteome of Mycobacterium tuberculosis.
    Electrophoresis. 2000 Nov;21(17):3740-56 PMID: 11271494
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.186.8.2487-2491.2004
Email
okkels@mpiib-berlin.mpg.de
Published
2004-04-00
Pages
2487-91
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC412125
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.186.8.2487-2491.2004 PMC Full Text View journal details
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