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PMID: 15060051 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

PhoP can activate its target genes in a PhoQ-independent manner.

Journal of bacteriology ·Vol. 186 ·No. 8 ·2004-04-00 ·Pages 2476-80

Lejona S, Castelli ME, Cabeza ML, Kenney LJ, García Véscovi E, Soncini FC

Abstract

The PhoP/PhoQ two-component system controls the extracellular magnesium depletion response in Salmonella enterica. Previous studies have shown that PhoP is unable to up-regulate its target genes in the absence of PhoQ function. In this work, we demonstrate that PhoP overexpression can substitute for PhoQ- and phosphorylation-dependent activation. Either a high concentration of PhoP or activation via phosphorylation stimulates PhoP self-association.

MeSH Terms
Bacterial Proteins/genetics,metabolism Gene Expression Regulation, Bacterial Genes, Bacterial Phosphorylation Protein Binding Salmonella enterica/genetics Transcription Factors/genetics,metabolism Transcription, Genetic
Chemicals
Bacterial Proteins PhoQ protein, Bacteria Transcription Factors PhoP protein, Bacteria
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lejona Sergio
Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, 2000 Rosario, Argentina.
Castelli María Eugenia
Cabeza María Laura
Kenney Linda J
García Véscovi Eleonora
Soncini Fernando C
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27 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.186.8.2476-2480.2004
Published
2004-04-00
Pages
2476-80
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC412160
Subset
IM
Grants
NIGMS NIH HHS · R01 GM058746 · United States
NIGMS NIH HHS · GM-58746 · United States
External Links
PubMed source DOI: 10.1128/JB.186.8.2476-2480.2004 PMC Full Text View journal details
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