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PMID: 15037769 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Definition of bases in 23S rRNA essential for ribosomal subunit association.

RNA (New York, N.Y.) ·Vol. 10 ·No. 4 ·2004-04-00 ·Pages 600-4

Maiväli U, Remme J

Abstract

The ribosome is a two-subunit molecular machine, sporting a working cycle that involves coordinated movements of the subunits. Recent structural studies of the 70S ribosome describe a rather large number of intersubunit contacts, some of which are dynamic during translocation. We set out to determine which intersubunit contacts are functionally indispensable for the association of ribosome subunits by using a modification interference approach. Modification of the N-1 position of A715, A1912, or A1918 in Escherichia coli 50S subunits is strongly detrimental to 70S ribosome formation. This result points to 23S rRNA helices 34 and 69, and thus bridges B2a and B4, as essential for ensuring stability of the 70S ribosome.

MeSH Terms
Deinococcus/chemistry Pseudouridine/metabolism RNA, Ribosomal, 23S/metabolism Ribosomes/genetics,metabolism Structure-Activity Relationship
Chemicals
RNA, Ribosomal, 23S Pseudouridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Maiväli Ulo
Department of Molecular Biology, Tartu University, Tartu, Estonia.
Remme Jaanus
References (25)
25 references, click to expand
  1. Formation of 70S ribosomes: large activation energy is required for the adaptation of exclusively the small ribosomal subunit.
    Biophys Chem. 2002 May 2;96(2-3):153-61 PMID: 12034437
  2. Ribosomal RNA pseudouridines and pseudouridine synthases.
    FEBS Lett. 2002 Mar 6;514(1):17-25 PMID: 11904174
  3. Ribosome interactions of aminoacyl-tRNA and elongation factor Tu in the codon-recognition complex.
    Nat Struct Biol. 2002 Nov;9(11):849-54 PMID: 12379845
  4. Structural basis of the ribosomal machinery for peptide bond formation, translocation, and nascent chain progression.
    Mol Cell. 2003 Jan;11(1):91-102 PMID: 12535524
  5. Study of the structural dynamics of the E coli 70S ribosome using real-space refinement.
    Cell. 2003 Jun 13;113(6):789-801 PMID: 12809609
  6. Locking and unlocking of ribosomal motions.
    Cell. 2003 Jul 11;114(1):123-34 PMID: 12859903
  7. Protection of specific sites in 16 S RNA from chemical modification by association of 30 S and 50 S ribosomes.
    J Mol Biol. 1977 Jan 5;109(1):131-49 PMID: 839533
  8. Protection of specific sites in 23 S and 5 S RNA from chemical modification by association of 30 S and 50 S ribosomes.
    J Mol Biol. 1979 Jun 5;130(4):421-32 PMID: 384002
  9. Mechanism of ribosomal subunit association: discrimination of specific sites in 16 S RNA essential for association activity.
    J Mol Biol. 1979 Jun 5;130(4):433-49 PMID: 384003
  10. Structural analysis of RNA using chemical and enzymatic probing monitored by primer extension.
    Methods Enzymol. 1988;164:481-9 PMID: 2468070
  11. Kinetic and thermodynamic parameters for tRNA binding to the ribosome and for the translocation reaction.
    J Biol Chem. 1992 Mar 5;267(7):4703-12 PMID: 1537853
  12. Identification of intermolecular RNA cross-links at the subunit interface of the Escherichia coli ribosome.
    Biochemistry. 1992 Mar 24;31(11):3004-11 PMID: 1372517
  13. The involvement of two distinct regions of 23 S ribosomal RNA in tRNA selection.
    J Mol Biol. 1995 Dec 15;254(5):838-47 PMID: 7500354
  14. Nucleotides in 16S rRNA protected by the association of 30S and 50S ribosomal subunits.
    J Mol Biol. 1999 Jan 8;285(1):97-105 PMID: 9878391
  15. Nucleotides in 23S rRNA protected by the association of 30S and 50S ribosomal subunits.
    J Mol Biol. 1999 Jan 8;285(1):107-13 PMID: 9878392
  16. Identification of an RNA-protein bridge spanning the ribosomal subunit interface.
    Science. 1999 Sep 24;285(5436):2133-6 PMID: 10497132
  17. Solution structure of the E. coli 70S ribosome at 11.5 A resolution.
    Cell. 2000 Mar 3;100(5):537-49 PMID: 10721991
  18. The complete atomic structure of the large ribosomal subunit at 2.4 A resolution.
    Science. 2000 Aug 11;289(5481):905-20 PMID: 10937989
  19. RNA tertiary interactions in the large ribosomal subunit: the A-minor motif.
    Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):4899-903 PMID: 11296253
  20. Crystal structure of the ribosome at 5.5 A resolution.
    Science. 2001 May 4;292(5518):883-96 PMID: 11283358
  21. Interaction of translation initiation factor 3 with the 30S ribosomal subunit.
    Mol Cell. 2001 Oct;8(4):855-64 PMID: 11684020
  22. Structure of the 80S ribosome from Saccharomyces cerevisiae--tRNA-ribosome and subunit-subunit interactions.
    Cell. 2001 Nov 2;107(3):373-86 PMID: 11701127
  23. High resolution structure of the large ribosomal subunit from a mesophilic eubacterium.
    Cell. 2001 Nov 30;107(5):679-88 PMID: 11733066
  24. Ribosome as a molecular machine.
    FEBS Lett. 2002 Mar 6;514(1):2-10 PMID: 11904172
  25. Accessibility of phosphates in domain I of 23 S rRNA in the ribosomal 50 S subunit as detected by R(P) phosphorothioates.
    Biochim Biophys Acta. 2002 Nov 13;1579(1):1-7 PMID: 12401213
Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1261/rna.5220504
Published
2004-04-00
Pages
600-4
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1370550
Subset
IM
External Links
PubMed source DOI: 10.1261/rna.5220504 PMC Full Text View journal details
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