Stathmin-tubulin interaction gradients in motile and mitotic cells.

Science (New York, N.Y.) ·Vol. 303 ·No. 5665 ·2004-03-19 ·Pages 1862-6

Niethammer P, Bastiaens P, Karsenti E

Abstract

The spatial organization of the microtubule cytoskeleton is thought to be directed by steady-state activity gradients of diffusible regulatory molecules. We visualized such intracellular gradients by monitoring the interaction between tubulin and a regulator of microtubule dynamics, stathmin, using a fluorescence resonance energy transfer (FRET) biosensor. These gradients were observed both during interphase in motile membrane protrusions and during mitosis around chromosomes, which suggests that a similar mechanism may contribute to the creation of polarized microtubule structures. These interaction patterns are likely to reflect phosphorylation of stathmin in these areas.

MeSH Terms

Animals Bacterial Proteins Binding Sites Cell Line Cell Movement Chromosomes/metabolism Cytosol/metabolism Fluorescence Resonance Energy Transfer Green Fluorescent Proteins Interphase Luminescent Proteins Microtubule Proteins Microtubules/metabolism,ultrastructure Mitosis Mutation Phosphoprotein Phosphatases/metabolism Phosphoproteins/genetics,metabolism Phosphorylation Protein Binding Recombinant Fusion Proteins/metabolism Spindle Apparatus/ultrastructure Stathmin Swine Tetradecanoylphorbol Acetate/pharmacology Transfection Tubulin/metabolism Xenopus Xenopus Proteins

Chemicals

Bacterial Proteins Luminescent Proteins Microtubule Proteins Phosphoproteins Recombinant Fusion Proteins STMN1 protein, Xenopus Stathmin Tubulin Xenopus Proteins citrine protein, bacteria Green Fluorescent Proteins Phosphoprotein Phosphatases Tetradecanoylphorbol Acetate

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Niethammer Philipp

European Molecular Biology Laboratory, EMBL, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.

Bastiaens Philippe

Karsenti Eric

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

1095-9203

DOI

10.1126/science.1094108

Published

2004-03-19

Pages

1862-6

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.1094108 View journal details

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Stathmin-tubulin interaction gradients in motile and mitotic cells.

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