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PMID: 15030759 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Tensin stabilizes integrin adhesive contacts in Drosophila.

Developmental cell ·Vol. 6 ·No. 3 ·2004-03-00 ·Pages 357-69

Torgler CN, Narasimha M, Knox AL, Zervas CG, Vernon MC, Brown NH

Abstract

We report the functional characterization of the Drosophila ortholog of tensin, a protein implicated in linking integrins to the cytoskeleton and signaling pathways. A tensin null was generated and is viable with wing blisters, a phenotype characteristic of loss of integrin adhesion. In tensin mutants, mechanical abrasion is required during wing expansion to cause wing blisters, suggesting that tensin strengthens integrin adhesion. The localization of tensin requires integrins, talin, and integrin-linked kinase. The N-terminal domain and C-terminal PTB domain of tensin provide essential recruitment signals. The intervening SH2 domain is not localized on its own. We suggest a model where tensin is recruited to sites of integrin adhesion via its PTB and N-terminal domains, localizing the SH2 domain so that it can interact with phosphotyrosine-containing proteins, which stabilize the integrin link to the cytoskeleton.

MeSH Terms

Animals Animals, Genetically Modified Cell Adhesion/genetics,physiology Cytoskeleton/metabolism Drosophila/genetics Embryo, Nonmammalian Focal Adhesion Protein-Tyrosine Kinases Gene Expression Gene Expression Regulation, Developmental Genes, Insect/genetics Green Fluorescent Proteins Immunohistochemistry/methods In Situ Hybridization/methods Insect Proteins/metabolism Integrins/genetics,physiology Larva Luminescent Proteins/metabolism Microfilament Proteins/genetics,physiology Models, Biological Mutation Phosphotyrosine/metabolism Polypyrimidine Tract-Binding Protein Protein Binding Protein Serine-Threonine Kinases/physiology Protein-Tyrosine Kinases/metabolism RNA, Messenger/biosynthesis Reverse Transcriptase Polymerase Chain Reaction/methods Stress, Physiological/genetics,metabolism Talin/physiology Tensins Tyrosine/metabolism Wings, Animal/embryology,metabolism src Homology Domains/physiology

Chemicals

Insect Proteins Integrins Luminescent Proteins Microfilament Proteins RNA, Messenger Talin Tensins Polypyrimidine Tract-Binding Protein Green Fluorescent Proteins Phosphotyrosine Tyrosine integrin-linked kinase Protein-Tyrosine Kinases Focal Adhesion Protein-Tyrosine Kinases Protein Serine-Threonine Kinases

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Torgler Catherine N

Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Anatomy, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, United Kingdom.

Narasimha Maithreyi

Knox Andrea L

Zervas Christos G

Vernon Matthew C

Brown Nicholas H

Article Info

Journal

Developmental cell

Abbr.

Dev Cell

ISSN

1534-5807

DOI

10.1016/s1534-5807(04)00055-3

Published

2004-03-00

Pages

357-69

Language

English

Region

United States

NLM ID

101120028

Subset

External Links

PubMed source DOI: 10.1016/s1534-5807(04)00055-3 View journal details

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Tensin stabilizes integrin adhesive contacts in Drosophila.

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