Molecular diversity of rumen methanogens from sheep in Western Australia.

Applied and environmental microbiology ·Vol. 70 ·No. 3 ·2004-03-00 ·Pages 1263-70

Wright AD, Williams AJ, Winder B, Christophersen CT, Rodgers SL, Smith KD

Abstract

The molecular diversity of rumen methanogens in sheep in Australia was investigated by using individual 16S rRNA gene libraries prepared from the rumen contents obtained from six merino sheep grazing pasture (326 clones), six sheep fed an oaten hay-based diet (275 clones), and five sheep fed a lucerne hay-based diet (132 clones). A total of 733 clones were examined, and the analysis revealed 65 phylotypes whose sequences (1,260 bp) were similar to those of cultivated methanogens belonging to the order Methanobrevibacter: Pasture-grazed sheep had more methanogen diversity than sheep fed either the oaten hay or lucerne hay diet. Methanobrevibacter strains SM9, M6, and NT7 accounted for over 90% of the total number of clones identified. M6 was more prevalent in grazing sheep, and SM9, despite being found in 16 of the 17 sheep, was more prevalent in sheep fed the lucerne-based diet. Five new species were identified. Two of these species exhibited very little sequence similarity to any cultivated methanogens and were found eight times in two of the six sheep that were grazing pasture. These unique sequences appear to represent a novel group of rumen archaea that are atypical for the rumen environment.

MeSH Terms

Animals Diet Gene Library Genetic Variation Methanobacteriales/genetics,isolation & purification Methanobrevibacter/genetics,isolation & purification Molecular Sequence Data Phylogeny RNA, Archaeal/genetics RNA, Ribosomal, 16S/genetics Rumen/microbiology Sheep/microbiology Western Australia

Chemicals

RNA, Archaeal RNA, Ribosomal, 16S

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Wright André-Denis G

CSIRO Livestock Industries, Centre for Environment and Life Sciences, Wembley, Western Australia 6913, Australia. andre-denis.wright@csiro.au

Williams Andrew J

Winder Barbara

Christophersen Claus T

Rodgers Sharon L

Smith Kellie D

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/AEM.70.3.1263-1270.2004

andre-denis.wright@csiro.au

Published

2004-03-00

Pages

1263-70

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC368393

Subset

Databases

GENBANK

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External Links

PubMed source DOI: 10.1128/AEM.70.3.1263-1270.2004 PMC Full Text View journal details

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Molecular diversity of rumen methanogens from sheep in Western Australia.

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