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PMID: 14990792 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Missing self-recognition of Ocil/Clr-b by inhibitory NKR-P1 natural killer cell receptors.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 101 ·No. 10 ·2004-03-09 ·Pages 3527-32

Carlyle JR, Jamieson AM, Gasser S, Clingan CS, Arase H, Raulet DH

Abstract

The NKR-P1 family of C-type lectin-like receptors are expressed on natural killer (NK) cells and NKT cells. We report the cloning and characterization of a cognate ligand for the inhibitory mouse NK receptors (NKR)-P1B and NKR-P1D (CD161b/d). The NKR-P1B/D ligand is osteoclast inhibitory lectin (Ocil), also known as Clr-b, a member of a previously cloned group of C-type lectin-related (Clr) proteins linked to the NKR-P1 receptors in the mouse NK gene complex (NKC). Expression of Ocil/Clr-b on mouse tumor cell lines inhibits NK cell-mediated killing. Inhibition is blocked with a new mAb (4A6) specific for Ocil/Clr-b. By using 4A6 mAb, we demonstrate that Ocil/Clr-b is displayed at high levels on nearly all hematopoietic cells, with the exception of erythrocytes, in a pattern that is similar to that of class I MHC molecules. Remarkably, Ocil/Clr-b is frequently down-regulated on mouse tumor cell lines, indicating a role for this receptor-ligand system in a new form of "missing self-recognition" of tumor cells.

MeSH Terms
Animals Antibodies, Monoclonal Antigens, Surface/genetics,metabolism Base Sequence Cell Line, Tumor Cloning, Molecular DNA, Complementary/genetics Killer Cells, Natural/immunology,metabolism Lectins, C-Type/genetics,metabolism Ligands Membrane Proteins/genetics,metabolism Mice Mice, Inbred BALB C Mice, Inbred C57BL Molecular Sequence Data NK Cell Lectin-Like Receptor Subfamily B Receptors, Immunologic/genetics,metabolism
Chemicals
Antibodies, Monoclonal Antigens, Surface DNA, Complementary Klrb1b protein, mouse Lectins, C-Type Ligands Membrane Proteins NK Cell Lectin-Like Receptor Subfamily B Ocil protein, mouse Receptors, Immunologic
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Carlyle James R
Department of Molecular and Cell Biology and Cancer Research Laboratory, University of California, 485 Life Sciences Addition, Berkeley, CA 94720, USA. james.carlyle@utoronto.ca
Jamieson Amanda M
Gasser Stephan
Clingan Christopher S
Arase Hisashi
Raulet David H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0308304101
Email
james.carlyle@utoronto.ca
Published
2004-03-09
Epub
2004-00-27
Pages
3527-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC373496
Subset
IM
Databases
GENBANK
AY320031
External Links
PubMed source DOI: 10.1073/pnas.0308304101 PMC Full Text View journal details
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