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PMID: 14990685 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Budding of PPxY-containing rhabdoviruses is not dependent on host proteins TGS101 and VPS4A.

Journal of virology ·Vol. 78 ·No. 6 ·2004-03-00 ·Pages 2657-65

Irie T, Licata JM, McGettigan JP, Schnell MJ, Harty RN

Abstract

Viral matrix proteins of several enveloped RNA viruses play important roles in virus assembly and budding and are by themselves able to bud from the cell surface in the form of lipid-enveloped, virus-like particles (VLPs). Three motifs (PT/SAP, PPxY, and YxxL) have been identified as late budding domains (L-domains) responsible for efficient budding. L-domains can functionally interact with cellular proteins involved in vacuolar sorting (VPS4A and TSG101) and endocytic pathways (Nedd4), suggesting involvement of these pathways in virus budding. Ebola virus VP40 has overlapping PTAP and PPEY motifs, which can functionally interact with TSG101 and Nedd4, respectively. As for vesicular stomatitis virus (VSV), a PPPY motif within M protein can interact with Nedd4. In addition, M protein has a PSAP sequence downstream of the PPPY motif, but the function of PSAP in budding is not clear. In this study, we compared L-domain functions between Ebola virus and VSV by constructing a chimeric M protein (M40), in which the PPPY motif of VSV M is replaced by the L domains of VP40. The budding efficiency of M40 was 10-fold higher than that of wild-type (wt) M protein. Overexpression of a dominant negative mutant of VPS4A or depletion of cellular TSG101 reduced the budding of only M40-containing VLPs but not that of wt M VLPs or live VSV. These findings suggest that the PSAP motif of M protein is not critical for budding and that there are fundamental differences between PTAP-containing viruses (Ebola virus and human immunodeficiency virus type 1) and PPPY-containing viruses (VSV and rabies virus) regarding their dependence on specific host factors for efficient budding.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Amino Acid Motifs Animals COS Cells Cell Line DNA-Binding Proteins/genetics,metabolism Ebolavirus/chemistry,growth & development Endosomal Sorting Complexes Required for Transport Humans Nucleoproteins/chemistry,metabolism Recombinant Fusion Proteins/genetics,metabolism Transcription Factors/genetics,metabolism Vesicular stomatitis Indiana virus/chemistry,growth & development Viral Core Proteins/chemistry,metabolism Viral Matrix Proteins/chemistry,genetics,metabolism Virion/metabolism Virus Assembly
Chemicals
DNA-Binding Proteins Endosomal Sorting Complexes Required for Transport M protein, Vesicular stomatitis virus Nucleoproteins Recombinant Fusion Proteins Transcription Factors Tsg101 protein Viral Core Proteins Viral Matrix Proteins nucleoprotein VP40, Ebola virus Adenosine Triphosphatases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Irie Takashi
Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Licata Jillian M
McGettigan James P
Schnell Matthias J
Harty Ronald N
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.78.6.2657-2665.2004
Published
2004-03-00
Pages
2657-65
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353768
Subset
IM
Grants
NIAID NIH HHS · T32 AI007324 · United States
NIAID NIH HHS · AI49153 · United States
NIAID NIH HHS · R21 AI049153 · United States
NIAID NIH HHS · T32-AI007324 · United States
NIAID NIH HHS · R01 AI049153 · United States
NIAID NIH HHS · AI46499 · United States
NIAID NIH HHS · R01 AI046499 · United States
NIAID NIH HHS · AI53393 · United States
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1128/jvi.78.6.2657-2665.2004 PMC Full Text View journal details
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