NGEP, a gene encoding a membrane protein detected only in prostate cancer and normal prostate.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 101 ·No. 9 ·2004-03-02 ·Pages 3059-64

Bera TK, Das S, Maeda H, Beers R, Wolfgang CD, Kumar V, Hahn Y, Lee B, Pastan I

Abstract

We identified a gene (NGEP) that is expressed only in prostate cancer and normal prostate. The two NGEP transcripts are 0.9 kb and 3.5 kb in size and are generated by a differential splicing event. The short variant (NGEP-S) is derived from four exons and encodes a 20-kDa intracellular protein. The long form (NGEP-L) is derived from 18 exons and encodes a 95-kDa protein that is predicted to contain seven-membrane-spanning regions. In situ hybridization shows that NGEP mRNA is localized in epithelial cells of normal prostate and prostate cancers. Immunocytochemical analysis of cells transfected with NGEP cDNAs containing a Myc epitope tag at the carboxyl terminus shows that the protein encoded by the short transcript is localized in the cytoplasm, whereas the protein encoded by the long transcript is present on the plasma membrane. Because of its selective expression in prostate cancer and its presence on the cell surface, NGEP-L is a promising target for the antibody-based therapies of prostate cancer.

MeSH Terms

Animals Anoctamins Base Sequence Cell Line DNA Primers Flow Cytometry Humans Male Membrane Proteins/genetics Mice Neoplasm Proteins/chemistry,genetics Organ Specificity Prostate/physiology Prostatic Neoplasms/chemistry,genetics Protein Biosynthesis Protein Conformation RNA, Messenger/analysis,genetics Rats Reverse Transcriptase Polymerase Chain Reaction Sequence Alignment Sequence Homology, Amino Acid Transcription, Genetic Transfection

Chemicals

ANO7 protein, human Anoctamins DNA Primers Membrane Proteins Neoplasm Proteins RNA, Messenger

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Bera Tapan K

Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Das Sudipto

Maeda Hiroshi

Beers Richard

Wolfgang Curt D

Kumar Vasantha

Hahn Yoonsoo

Lee Byungkook

Pastan Ira

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.0308746101

Published

2004-03-02

Epub

2004-00-23

Pages

3059-64

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC365744

Subset

External Links

PubMed source DOI: 10.1073/pnas.0308746101 PMC Full Text View journal details

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NGEP, a gene encoding a membrane protein detected only in prostate cancer and normal prostate.

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