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PMID: 14968301 Published · ppublish English Comparative Study Journal Article

One hundred and one new microsatellite loci derived from ESTs (EST-SSRs) in bread wheat.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik ·Vol. 108 ·No. 7 ·2004-05-00 ·Pages 1392-400

Gao LF, Jing RL, Huo NX, Li Y, Li XP, Zhou RH, Chang XP, Tang JF, Ma ZY, Jia JZ

Abstract

Four hundred and seventy-eight microsatellite markers derived from expressed sequence tags (EST-SSRs) were screened among three mapping populations (W-7984xOpata 85, WOpop; LumaixHanxuan, LHpop; WenmaixShanhongmai, WSpop). The number of polymorphic EST-SSR primer pairs found in WOpop, LHpop and WSpop was 92, 58 and 29 respectively. A total of 101 EST-SSR loci amplified from 88 primer sets were distributed over the 20 chromosomes of the reference maps (no markers were located on chromosome 4B). These 101 mapped EST-SSR markers add to the existing 450 microsatellite loci previously mapped in bread wheat. Seventy-four of the 101 loci showed significant similarities to known genes, including 24 genes involved in metabolism, 4 in cellular structures, 9 in stress resistance, 12 in transcription, 2 in development, 2 transporters and 21 storage proteins. Besides gliadin and glutenin, most of the 53 genes with putative functions were mapped for the first time by EST-SSR markers in bread wheat. Sequence alignment of the mapped wheat EST-SSR loci allowed tentative assignment of functionality to the other members of grasses family. Colinearity combined with homology information offers an attractive approach to comparative genomics.

MeSH Terms
Chromosome Mapping Crosses, Genetic Expressed Sequence Tags Lod Score Microsatellite Repeats/genetics Phenotype Polymorphism, Genetic Triticum/genetics
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Gao L F
Key Laboratory of Crop Germplasm and Biotechnology, Ministry of Agriculture, Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, 100081 Beijing, China.
Jing R L
Huo N X
Li Y
Li X P
Zhou R H
Chang X P
Tang J F
Ma Z Y
Jia J Z
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Article Info
Journal
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Abbr.
Theor Appl Genet
ISSN
0040-5752
DOI
10.1007/s00122-003-1554-z
Published
2004-05-00
Epub
2004-00-14
Pages
1392-400
Language
English
Region
Germany
NLM ID
0145600
Subset
IM
External Links
PubMed source DOI: 10.1007/s00122-003-1554-z View journal details
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