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PMID: 14769929 Published · ppublish English Journal Article

The outer plastid envelope protein Oep16: role as precursor translocase in import of protochlorophyllide oxidoreductase A.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 101 ·No. 7 ·2004-02-17 ·Pages 2203-8

Reinbothe S, Quigley F, Springer A, Schemenewitz A, Reinbothe C

Abstract

A 16-kDa plastid envelope protein was identified by chemical crosslinking that interacts with the precursor of NADPH:protochlorophyllide oxdidoreductase A (pPORA) during its posttranslational import into isolated barley chloroplasts. Protein purification and subsequent protein sequencing showed that the 16-kDa protein is an ortholog of a previously identified outer plastid envelope protein, Oep16. A protein of identical size was present in barley etioplasts and interacted with pPORA. Similar 16-kDa protein-dependent crosslink products of pPORA were detected in wheat, pea, and Arabidopsis chloroplasts. Database analyses revealed that the 16-kDa protein belongs to a family of preprotein and amino acid transporters found in free-living bacteria and endosymbiotic mitochondria and chloroplasts. Antibodies raised against the 16-kDa protein inhibited import of pPORA, highlighting its role in protein import.

MeSH Terms
Amino Acid Sequence Chloroplast Proteins Cross-Linking Reagents Evolution, Molecular Intracellular Membranes/chemistry,metabolism Ion Channels/chemistry,metabolism Membrane Transport Proteins/chemistry,metabolism Molecular Sequence Data Molecular Weight Oxidoreductases Acting on CH-CH Group Donors/metabolism Plant Cells Plant Proteins/chemistry,metabolism Plants/chemistry,enzymology,metabolism Plastids/chemistry,metabolism Protein Binding Protein Precursors/chemistry,metabolism Protein Transport
Chemicals
Chloroplast Proteins Cross-Linking Reagents Ion Channels Membrane Transport Proteins Plant Proteins Protein Precursors Oxidoreductases Acting on CH-CH Group Donors protochlorophyllide reductase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Reinbothe Steffen
Université Joseph Fourier et Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5575, BP53, F-38041 Grenoble Cedex 9, France. steffen.reinbothe@ujf-grenoble.fr
Quigley Françoise
Springer Armin
Schemenewitz Andreas
Reinbothe Christiane
References (43)
43 references, click to expand
  1. Protein Import into and Sorting inside the Chloroplast Are Independent Processes.
    Plant Cell. 1990 May;2(5):479-494 PMID: 12354964
  2. Signal peptide analogs derived from two chloroplast precursors interact with the signal recognition system of the chloroplast envelope.
    J Biol Chem. 1991 Feb 15;266(5):3335-42 PMID: 1993706
  3. An Arabidopsis mutant defective in the plastid general protein import apparatus.
    Science. 1998 Oct 2;282(5386):100-3 PMID: 9756470
  4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  5. A second, substrate-dependent site of protein import into chloroplasts.
    Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9795-800 PMID: 10920193
  6. Identification of plastid envelope proteins required for import of protochlorophyllide oxidoreductase A into the chloroplast of barley.
    Proc Natl Acad Sci U S A. 2004 Feb 17;101(7):2197-202 PMID: 14769934
  7. Direct probing of the interaction between the signal sequence of nascent preprolactin and the signal recognition particle by specific cross-linking.
    J Cell Biol. 1987 Feb;104(2):201-8 PMID: 3643215
  8. Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation.
    Cell. 1996 Nov 15;87(4):721-32 PMID: 8929540
  9. Synthetic analogues of a transit peptide inhibit binding or translocation of chloroplastic precursor proteins.
    J Biol Chem. 1991 Jun 25;266(18):11882-9 PMID: 2050684
  10. Substrate-dependent and organ-specific chloroplast protein import in planta.
    Plant Cell. 2004 Jan;16(1):88-98 PMID: 14688290
  11. NADPH:Protochlorophyllide oxidoreductase uses the general import route into chloroplasts.
    Biol Chem. 2000 Dec;381(12):1263-7 PMID: 11209762
  12. Translocation arrest of an intramitochondrial sorting signal next to Tim11 at the inner-membrane import site.
    Nature. 1996 Dec 12;384(6609):585-8 PMID: 8955275
  13. Isolation and characterization of an amino acid-selective channel protein present in the chloroplastic outer envelope membrane.
    Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):9504-9 PMID: 9256512
  14. Identification of the protein import components of the rat mitochondrial inner membrane, rTIM17, rTIM23, and rTIM44.
    J Biochem. 1998 Apr;123(4):722-32 PMID: 9538267
  15. A plastid enzyme arrested in the step of precursor translocation in vivo.
    Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):12026-30 PMID: 11607713
  16. High expression level of a gene coding for a chloroplastic amino acid selective channel protein is correlated to cold acclimation in cereals.
    Plant Mol Biol. 1999 Sep;41(2):233-43 PMID: 10579490
  17. Control of gene expression during higher plant chloroplast biogenesis. Protein synthesis and transcript levels of psbA, psaA-psaB, and rbcL in dark-grown and illuminated barley seedlings.
    J Biol Chem. 1987 Mar 25;262(9):4341-8 PMID: 3558409
  18. Two routes of chlorophyllide synthesis that are differentially regulated by light in barley (Hordeum vulgare L.).
    Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3254-8 PMID: 7724548
  19. The chloroplastic protein import machinery contains a Rieske-type iron-sulfur cluster and a mononuclear iron-binding protein.
    EMBO J. 1997 Dec 15;16(24):7342-50 PMID: 9405363
  20. Substrate-dependent transport of the NADPH:protochlorophyllide oxidoreductase into isolated plastids.
    Plant Cell. 1995 Feb;7(2):161-72 PMID: 7756827
  21. Two components of the chloroplast protein import apparatus, IAP86 and IAP75, interact with the transit sequence during the recognition and translocation of precursor proteins at the outer envelope.
    J Cell Biol. 1996 Jul;134(2):315-27 PMID: 8707818
  22. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  23. The major protein import receptor of plastids is essential for chloroplast biogenesis.
    Nature. 2000 Jan 13;403(6766):203-7 PMID: 10646606
  24. Gateway to the chloroplast.
    Nature. 2000 Jan 13;403(6766):148-9 PMID: 10646583
  25. Isolation of components of the chloroplast protein import machinery.
    Science. 1994 Nov 11;266(5187):1007-12 PMID: 7973649
  26. Molecular biology of chloroplast biogenesis: gene expression, protein import and intraorganellar sorting.
    Cell Mol Life Sci. 2001 Mar;58(3):420-33 PMID: 11315189
  27. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  28. Initial binding of preproteins involving the Toc159 receptor can be bypassed during protein import into chloroplasts.
    Plant Physiol. 2000 Mar;122(3):813-22 PMID: 10712545
  29. The preprotein translocation channel of the outer membrane of mitochondria.
    Cell. 1998 Jun 12;93(6):1009-19 PMID: 9635430
  30. The preprotein translocase of the mitochondrial inner membrane: function and evolution.
    J Mol Biol. 1999 Feb 12;286(1):105-20 PMID: 9931253
  31. Travelling of proteins through membranes: translocation into chloroplasts.
    Planta. 2000 Sep;211(4):449-56 PMID: 11030543
  32. Protein import and routing systems of chloroplasts.
    Plant Cell. 1999 Apr;11(4):557-70 PMID: 10213778
  33. Versatility of the mitochondrial protein import machinery.
    Nat Rev Mol Cell Biol. 2001 May;2(5):339-49 PMID: 11331908
  34. Multiple sequence alignment with Clustal X.
    Trends Biochem Sci. 1998 Oct;23(10):403-5 PMID: 9810230
  35. TreeView: an application to display phylogenetic trees on personal computers.
    Comput Appl Biosci. 1996 Aug;12(4):357-8 PMID: 8902363
  36. Analysis of the interactions of preproteins with the import machinery over the course of protein import into chloroplasts.
    J Cell Biol. 1997 Dec 29;139(7):1677-85 PMID: 9412463
  37. Two NADPH:Protochlorophyllide Oxidoreductases in Barley: Evidence for the Selective Disappearance of PORA during the Light-Induced Greening of Etiolated Seedlings.
    Plant Cell. 1995 Nov;7(11):1933-1940 PMID: 12242364
  38. Reconstitution of a chloroplast protein import channel.
    EMBO J. 1997 Dec 15;16(24):7351-60 PMID: 9405364
  39. [37] Reaction of protein sulfhydryl groups with Ellman's reagent.
    Methods Enzymol. 1972;25:457-64 PMID: 23014426
  40. Identification of NADPH:protochlorophyllide oxidoreductases A and B: a branched pathway for light-dependent chlorophyll biosynthesis in Arabidopsis thaliana.
    Plant Physiol. 1995 Aug;108(4):1505-17 PMID: 7659751
  41. The interaction of oxidized glutathione, cystamine monosulfoxide, and tetrathionate with the-SH groups of rabbit muscle D-glyceraldehyde 3-phosphate dehydrogenase.
    J Biol Chem. 1962 Apr;237:1356-62 PMID: 14486599
  42. Regulation of chloroplast protein import through a protochlorophyllide-responsive transit peptide.
    Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8890-4 PMID: 11038562
  43. Targeting of proteins to the outer envelope membrane uses a different pathway than transport into chloroplasts.
    Plant Cell. 1991 Jul;3(7):709-17 PMID: 1841725
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0301962101
Email
steffen.reinbothe@ujf-grenoble.fr
Published
2004-02-17
Epub
2004-00-09
Pages
2203-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC357075
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0301962101 PMC Full Text View journal details
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