The carbohydrates of influenza virus. II. Gas chromatographic analysis of glycopeptides derived from viral glycoproteins and mucopolysaccharides.

Archives of virology ·Vol. 56 ·No. 3 ·1978-00-00 ·Pages 251-5

Schwarz RT, Fournet B, Montreuil J, Rott R, Klenk HD

Abstract

Two carbohydrate fractions can be obtained from egg-grown influenza virus after Pronase digestion followed by gel chromatography. One fraction contains glycopeptides (MW ca. 2000--2600) which represent the carbohydrate side chains of the viral glycoproteins. The constituent sugars of this material are fucose, galactose, glucosamine, and mannose, and their position within the side chain has been partially elucidated by methylation studies. The other fraction (MW greatest than 6000) appears to be mucopolysaccharide and is composed of fucose, galactose, glucose, galactosamine, and glucosamine.

MeSH Terms

Chromatography, Gas Fucose/analysis Galactosamine/analysis Galactose/analysis Glucosamine/analysis Glucose/analysis Glycopeptides/analysis Glycoproteins/analysis Glycosaminoglycans/analysis Influenza A virus/analysis Mannose/analysis

Chemicals

Glycopeptides Glycoproteins Glycosaminoglycans Fucose Galactosamine Glucose Glucosamine Mannose Galactose

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Schwarz R T

Fournet B

Montreuil J

Rott R

Klenk H D

References (11)

11 references, click to expand

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Carbohydrates of influenza virus. I. Glycopeptides derived from viral glycoproteins after labeling with radioactive sugars.
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Analysis of monosaccharides by gas-liquid chromatography of the O-methyl glycosides as trifluoroacetate derivatives. Application to glycoproteins and glycolipids.
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Article Info

Journal

Archives of virology

Abbr.

Arch Virol

ISSN

0304-8608

DOI

10.1007/BF01317854

Published

1978-00-00

Pages

251-5

Language

English

Region

Austria

NLM ID

7506870

Subset

External Links

PubMed source DOI: 10.1007/BF01317854 View journal details

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The carbohydrates of influenza virus. II. Gas chromatographic analysis of glycopeptides derived from viral glycoproteins and mucopolysaccharides.

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