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PMID: 1476429 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The beta-mannanase from "Caldocellum saccharolyticum" is part of a multidomain enzyme.

Applied and environmental microbiology ·Vol. 58 ·No. 12 ·1992-12-00 ·Pages 3864-7

Gibbs MD, Saul DJ, Lüthi E, Bergquist PL

Abstract

The complete sequence of a beta-mannanase gene from an anaerobic extreme thermophile was determined, and it shows that the expressed protein consists of two catalytic domains and two binding domains separated by spacer regions rich in proline and threonine residues. The amino-terminal catalytic domain has beta-mannanase activity, and the carboxy-terminal domain acts as an endoglucanase. Neither domain shows homology with any other cellulase or hemicellulase sequence at the nucleic acid or protein level.

Related Genes

celA celB manA

MeSH Terms

Amino Acid Sequence Bacteria, Anaerobic/enzymology,genetics Base Sequence Cellulase/genetics DNA, Bacterial/genetics Mannosidases/genetics Molecular Sequence Data Multienzyme Complexes/genetics Protein Conformation Sequence Homology, Amino Acid beta-Mannosidase

Chemicals

DNA, Bacterial Multienzyme Complexes Mannosidases beta-Mannosidase Cellulase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Gibbs M D

Department of Cellular & Molecular Biology, University of Auckland, New Zealand.

Saul D J

Lüthi E

Bergquist P L

References (10)

10 references, click to expand

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Two Bacillus beta-mannanases having different COOH termini are produced in Escherichia coli carrying pMAH5.
Appl Environ Microbiol. 1989 Dec;55(12):3178-83 PMID: 2694961
Structure of a Bacillus subtilis endo-beta-1,4-glucanase gene.
Nucleic Acids Res. 1986 Nov 25;14(22):9159-70 PMID: 3024130
Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen.
Appl Environ Microbiol. 1982 Apr;43(4):777-80 PMID: 7081984
Nucleotide sequence of a cellulase gene of Bacillus subtilis.
Eur J Biochem. 1987 Apr 15;164(2):317-20 PMID: 3106035
Hemicellulases of Bacillus species: preliminary comparative studies on production and properties of mannanases and galactanases.
J Appl Bacteriol. 1990 Mar;68(3):253-61 PMID: 2111303
celB, a gene coding for a bifunctional cellulase from the extreme thermophile "Caldocellum saccharolyticum".
Appl Environ Microbiol. 1990 Oct;56(10):3117-24 PMID: 2126700
Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties.
Nucleic Acids Res. 1988 Aug 11;16(15):7583-600 PMID: 2970625
Inducible expression vectors incorporating the Escherichia coli atpE translational initiation region.
Gene. 1987;52(2-3):279-83 PMID: 3038690
Cloning, sequence analysis, and expression in Escherichia coli of a gene coding for a beta-mannanase from the extremely thermophilic bacterium "Caldocellum saccharolyticum".
Appl Environ Microbiol. 1991 Mar;57(3):694-700 PMID: 2039230

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.58.12.3864-3867.1992

Published

1992-12-00

Pages

3864-7

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC183195

Subset

Databases

GENBANK

L01257, M55230, M55231, M55232, M55233, M55234, M80353, M80354, X58725, X58726

External Links

PubMed source DOI: 10.1128/aem.58.12.3864-3867.1992 PMC Full Text View journal details

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The beta-mannanase from "Caldocellum saccharolyticum" is part of a multidomain enzyme.

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