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PMID: 14745001 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Noninvasive two-photon imaging reveals retinyl ester storage structures in the eye.

The Journal of cell biology ·Vol. 164 ·No. 3 ·2004-02-02 ·Pages 373-83

Imanishi Y, Batten ML, Piston DW, Baehr W, Palczewski K

Abstract

Visual sensation in vertebrates is triggered when light strikes retinal photoreceptor cells causing photoisomerization of the rhodopsin chromophore 11-cis-retinal to all-trans-retinal. The regeneration of preillumination conditions of the photoreceptor cells requires formation of 11-cis-retinal in the adjacent retinal pigment epithelium (RPE). Using the intrinsic fluorescence of all-trans-retinyl esters, noninvasive two-photon microscopy revealed previously uncharacterized structures (6.9 +/- 1.1 microm in length and 0.8 +/- 0.2 microm in diameter) distinct from other cellular organelles, termed the retinyl ester storage particles (RESTs), or retinosomes. These structures form autonomous all-trans-retinyl ester-rich intracellular compartments distinct from other organelles and colocalize with adipose differentiation-related protein. As demonstrated by in vivo experiments using wild-type mice, the RESTs participate in 11-cis-retinal formation. RESTs accumulate in Rpe65-/- mice incapable of carrying out the enzymatic isomerization, and correspondingly, are absent in the eyes of Lrat-/- mice deficient in retinyl ester synthesis. These results indicate that RESTs located close to the RPE plasma membrane are essential components in 11-cis-retinal production.

MeSH Terms
Acyltransferases/genetics,metabolism Animals Carrier Proteins Cytoplasmic Vesicles/chemistry,metabolism Diterpenes Esters/analysis Eye/chemistry,metabolism,ultrastructure Eye Proteins Membrane Proteins/metabolism Mice Mice, Inbred Strains Mice, Knockout Microscopy, Fluorescence/methods Models, Molecular Molecular Structure Perilipin-2 Pigment Epithelium of Eye/cytology,metabolism Proteins/genetics,metabolism Retinaldehyde/administration & dosage,chemistry,metabolism Visual Perception/physiology Vitamin A/chemistry,metabolism cis-trans-Isomerases
Chemicals
Carrier Proteins Diterpenes Esters Eye Proteins Membrane Proteins Perilipin-2 Plin2 protein, mouse Proteins Vitamin A 9-cis-retinal Acyltransferases lecithin-retinol acyltransferase retinoid isomerohydrolase cis-trans-Isomerases Retinaldehyde
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Imanishi Yoshikazu
Department of Ophthalmology, University of Washington, 1959 NE Pacific St., Box 356485, Seattle, WA 98195-6485, USA.
Batten Matthew L
Piston David W
Baehr Wolfgang
Palczewski Krzysztof
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200311079
Published
2004-02-02
Epub
2004-00-26
Pages
373-83
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC1360214
Subset
IM
Grants
NEI NIH HHS · R01 EY009339 · United States
NEI NIH HHS · EY08123 · United States
NIDDK NIH HHS · DK53434 · United States
NIDDK NIH HHS · R01 DK053434 · United States
NEI NIH HHS · EY09339 · United States
NEI NIH HHS · EY13385 · United States
NEI NIH HHS · R01 EY008123 · United States
NEI NIH HHS · R01 EY013385 · United States
External Links
PubMed source DOI: 10.1083/jcb.200311079 PMC Full Text View journal details
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