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题目:: Role of phenytoin in wound healing: microarray analysis of early transcriptional responses in human dermal fibroblasts.
作者:: Swamy(S M K),Tan(P),Zhu(Y Z),Lu(J),Achuth(H N),Moochhala(S)
状态:: 发布时间2004-01-26 , 更新时间 2013-11-21
期刊:: Biochem Biophys Res Commun
摘要:: Wound healing is a complex process involving a number of related genes and receptors. Using cDNA microarrays, we explored the global gene expression profile of phenytoin (20microg/ml) induced changes to human dermal fibroblasts. Microarray data analysis revealed approximately 1500 genes were differentially expressed by 2.5-fold. At 3, 6, 12, and 24h, the transcripts of the major growth factors involved in wound healing and their receptors were increased. This was further confirmed by RT-PCR. Genes encoding other proteins with roles in signal transduction (NFkappaB), extracellular matrix (MMP1) including type I collagen, fibronectin, and laminin were strongly induced at 6h and onwards. Genes involved in cell cycle regulation (CCND1 and CDKN1A) were down-regulated consistent with our finding that phenytoin per se did not have cell proliferation activity. Notably, phenytoin accelerates the autocrine and paracrine activity of growth factors by up-regulating the related receptors.
语言:: eng
DOI:

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