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PMID: 14739654 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genetic vaccination of mice with plasmids encoding the NS1 non-structural protein from tick-borne encephalitis virus and dengue 2 virus.

Virus genes ·Vol. 28 ·No. 1 ·2004-01-00 ·Pages 85-97

Timofeev AV, Butenko VM, Stephenson JR

Abstract

Although there is a safe, inexpensive and efficacious vaccine against yellow fever, vaccination against other flavivirus diseases is less successful. There is no licensed vaccine against dengue fever and current vaccines against tick-borne encephalitis (TBE) and Japanese encephalitis are expensive and require several injections. Furthermore novel vaccines containing only virus envelope proteins may raise fears over antibody mediated enhancement (ADE) of disease. Here we report the successful use of genetic vaccination against TBE in an experimental animal model using a plasmid containing the coding sequence of a non-structural protein (NS1). Such vaccines would provide inexpensive protection against disease, without raising concerns over inducing ADE on subsequent exposure to heterotypic infectious virus. Attempts to generate chaemeric plasmids to protect against both TBE and dengue fever were less successful. Although these chaemeric plasmids directed the synthesis and secretion of the virus NS1 protein normally, no protection was observed against either TBE or dengue fever.

MeSH Terms
Amino Acid Sequence Animals Chlorocebus aethiops Dengue Virus/genetics,immunology Dimerization Encephalitis Viruses, Tick-Borne/genetics,immunology Mice Molecular Sequence Data Plasmids Protein Structure, Secondary Vaccines, DNA Vero Cells Viral Nonstructural Proteins/genetics,immunology
Chemicals
NS1 protein, Flavivirus Vaccines, DNA Viral Nonstructural Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Timofeev A V
Chumakov Institute of Poliomyelitis and Viral Encephalitis RAMS, Moscow Region, Russia.
Butenko V M
Stephenson J R
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Article Info
Journal
Virus genes
Abbr.
Virus Genes
ISSN
0920-8569
DOI
10.1023/B:VIRU.0000012266.04871.ce
Published
2004-01-00
Pages
85-97
Language
English
Region
United States
NLM ID
8803967
Subset
IM
External Links
PubMed source DOI: 10.1023/B:VIRU.0000012266.04871.ce View journal details
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