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PMID: 1472014 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Targeting aequorin to the endoplasmic reticulum of living cells.

Biochemical and biophysical research communications ·Vol. 189 ·No. 2 ·1992-12-15 ·Pages 1008-16

Kendall JM, Dormer RL, Campbell AK

Abstract

The photoprotein aequorin has been engineered with an ER targeting sequence at the N-terminus, with and without KDEL at the C-terminus, so that it locates in the ER-secretory pathway. For the first time the free Ca2+ has been quantified inside the ER and shown to be 5-20 times that in the cytosol. In COS cells free Ca2+ in the ER ranged from 1-5mM at 37 degrees C, decreasing 2-5-fold within 1 min of exposure to the Ca2+ ionophore ionomycin in the absence of external Ca2+.

MeSH Terms

Aequorin/genetics,metabolism Amino Acid Sequence Animals Base Sequence Calcium/metabolism,pharmacology Calcium-Binding Proteins/genetics Calreticulin Cell Line Cytosol/metabolism DNA/genetics,isolation & purification Endoplasmic Reticulum/drug effects,metabolism Ionomycin/pharmacology Microsomes/metabolism Molecular Sequence Data Oligodeoxyribonucleotides Pancreas/metabolism Polymerase Chain Reaction Protein Engineering Protein Sorting Signals/genetics RNA, Messenger/genetics,metabolism Rabbits Recombinant Proteins/metabolism Reticulocytes/metabolism Scyphozoa/genetics Transfection beta-Lactamases/genetics

Chemicals

Calcium-Binding Proteins Calreticulin Oligodeoxyribonucleotides Protein Sorting Signals RNA, Messenger Recombinant Proteins Aequorin Ionomycin DNA beta-Lactamases Calcium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kendall J M

Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff, UK.

Dormer R L

Campbell A K

Article Info

Journal

Biochemical and biophysical research communications

Abbr.

Biochem Biophys Res Commun

ISSN

0006-291X

DOI

10.1016/0006-291x(92)92304-g

Published

1992-12-15

Pages

1008-16

Language

English

Region

United States

NLM ID

0372516

Subset

External Links

PubMed source DOI: 10.1016/0006-291x(92)92304-g View journal details

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