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PMID: 14699088 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

i-SNAREs: inhibitory SNAREs that fine-tune the specificity of membrane fusion.

The Journal of cell biology ·Vol. 164 ·No. 1 ·2004-01-05 ·Pages 79-88

Varlamov O, Volchuk A, Rahimian V, Doege CA, Paumet F, Eng WS, Arango N, Parlati F, Ravazzola M, Orci L, Söllner TH, Rothman JE

Abstract

A new functional class of SNAREs, designated inhibitory SNAREs (i-SNAREs), is described here. An i-SNARE inhibits fusion by substituting for or binding to a subunit of a fusogenic SNAREpin to form a nonfusogenic complex. Golgi-localized SNAREs were tested for i-SNARE activity by adding them as a fifth SNARE together with four other SNAREs that mediate Golgi fusion reactions. A striking pattern emerges in which certain subunits of the cis-Golgi SNAREpin function as i-SNAREs that inhibit fusion mediated by the trans-Golgi SNAREpin, and vice versa. Although the opposing distributions of the cis- and trans-Golgi SNAREs themselves could provide for a countercurrent fusion pattern in the Golgi stack, the gradients involved would be strongly sharpened by the complementary countercurrent distributions of the i-SNAREs.

MeSH Terms
Binding Sites/physiology Golgi Apparatus/metabolism,ultrastructure Intracellular Membranes/chemistry,metabolism,ultrastructure Membrane Fusion/physiology Membrane Proteins/classification,genetics,metabolism Protein Binding/physiology Protein Subunits/metabolism Protein Transport/physiology SNARE Proteins Signal Transduction/physiology Vesicular Transport Proteins trans-Golgi Network/physiology
Chemicals
Membrane Proteins Protein Subunits SNARE Proteins Vesicular Transport Proteins
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Varlamov Oleg
Department of Cellular Biochemistry and Biophysics, Memorial Sloan-Kettering Cancer Center, 1275 York Ave., Box 251, New York, NY 10021, USA.
Volchuk Allen
Rahimian Vahid
Doege Claudia A
Paumet Fabienne
Eng William S
Arango Nancy
Parlati Francesco
Ravazzola Mariella
Orci Lelio
Söllner Thomas H
Rothman James E
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200307066
Published
2004-01-05
Epub
2003-00-29
Pages
79-88
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2171956
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200307066 PMC Full Text View journal details
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