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PMID: 14696356 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Defined conditions for neural commitment and differentiation.

Methods in enzymology ·Vol. 365 ·2003-00-00 ·Pages 327-41

Ying QL, Smith AG

Abstract

The efficiency of monolayer differentiation establishes that commitment of ES cells to a neural fate needs neither multicellular aggregation nor extrinsic inducers. The entire process by which pluripotent ES cells acquire neural specification can be visualized and recorded at the level of individual colonies. Furthermore this simple culture system is amenable to cellular and molecular dissection, promising to yield new insights into the mechanism underlying neural determination in mammals and perhaps to deliver the goal of "directed" homogeneous differentiation of ES cells.

MeSH Terms
Animals Cell Culture Techniques/methods Cell Death Cell Differentiation Cell Lineage Cell Separation Cells, Cultured Culture Media/pharmacology Culture Media, Serum-Free/pharmacology DNA-Binding Proteins/metabolism Flow Cytometry Green Fluorescent Proteins HMGB Proteins High Mobility Group Proteins/metabolism Luminescent Proteins/metabolism Mice Microscopy, Phase-Contrast Neurons/cytology Nuclear Proteins/metabolism SOXB1 Transcription Factors Transcription Factors
Chemicals
Culture Media Culture Media, Serum-Free DNA-Binding Proteins HMGB Proteins High Mobility Group Proteins Luminescent Proteins Nuclear Proteins SOXB1 Transcription Factors Sox1 protein, mouse Sox2 protein, mouse Transcription Factors Green Fluorescent Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ying Qi-Long
Institute for Stem Cell Research, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh, Scotland, EH9 3JQ, UK.
Smith Austin G
Article Info
Journal
Methods in enzymology
Abbr.
Methods Enzymol
ISSN
0076-6879
DOI
10.1016/s0076-6879(03)65023-8
Published
2003-00-00
Pages
327-41
Language
English
Region
United States
NLM ID
0212271
Subset
IM
External Links
PubMed source DOI: 10.1016/s0076-6879(03)65023-8 View journal details
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