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PMID: 14688296 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The ASK1 and ASK2 genes are essential for Arabidopsis early development.

The Plant cell ·Vol. 16 ·No. 1 ·2004-01-00 ·Pages 5-20

Liu F, Ni W, Griffith ME, Huang Z, Chang C, Peng W, Ma H, Xie D

Abstract

The requirement of CUL1 for Arabidopsis embryogenesis suggests that Skp1-CUL1-F-box protein (SCF) complexes play important roles during embryo development. Among the 21 Arabidopsis Skp1-like genes (ASKs), it is unknown which ASK gene(s) is essential for embryo development. In this study, we demonstrate a vital role for ASK1 and ASK2 in Arabidopsis embryogenesis and postembryonic development through analysis of the ask1 ask2 double mutant. Our detailed analysis indicates that the double mutations in both ASK1 and ASK2 affect cell division and cell expansion/elongation and cause a developmental delay during embryogenesis and lethality in seedling growth. The expression patterns of ASK1 and ASK2 were examined further and found to be consistent with their roles in embryogenesis and seedling development. We propose that mutations in ASK1 and ASK2 abolish all of the ASK1- and ASK2-based SCF and non-SCF complexes, resulting in alteration of gene expression and leading to defects in growth and development.

MeSH Terms
Arabidopsis/embryology,genetics Arabidopsis Proteins/genetics,metabolism Cell Division/genetics Culture Techniques Cyclin D3 Cyclins/genetics,metabolism Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genetic Complementation Test Mutation Plant Roots/genetics,growth & development SKP Cullin F-Box Protein Ligases Seeds/genetics,growth & development Ubiquitin-Protein Ligase Complexes/genetics,metabolism
Chemicals
ASK1 protein, Arabidopsis Arabidopsis Proteins Cyclin D3 Cyclins Ubiquitin-Protein Ligase Complexes ASK2 protein, Arabidopsis SKP Cullin F-Box Protein Ligases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Liu Fuquan
Laboratory of Plant Signal Transduction, Institute of Molecular and Cell Biology, 117609 Republic of Singapore.
Ni Weimin
Griffith Megan E
Huang Zhiyuan
Chang Changqing
Peng Wen
Ma Hong
Xie Daoxin
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.017772
Published
2004-01-00
Epub
2003-00-19
Pages
5-20
Language
English
Region
England
NLM ID
9208688
PMCID
PMC301391
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.017772 PMC Full Text View journal details
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