A DNA fragment homologous to F1-ATPase beta subunit was amplified from genomic DNA of Methanosarcina barkeri. Indication of an archaebacterial F-type ATPase.

FEBS letters ·Vol. 314 ·No. 3 ·1992-12-21 ·Pages 207-10

Sumi M, Sato MH, Denda K, Date T, Yoshida M

Abstract

A 490 bp DNA fragment was amplified from Methanosarcina barkeri genomic DNA by the polymerase chain reaction (PCR) using oligonucleotide primers designed based on conserved amino acid sequences of the F1-ATPase beta subunits. The amino acid sequence deduced from the DNA sequence of this fragment was highly homologous to a portion of the F1-ATPase beta subunit. This indicates that this archaebacterium has a gene of F-type ATPase in addition to a gene of V-type ATPase.

MeSH Terms

Amino Acid Sequence Base Sequence Blotting, Southern DNA, Bacterial/genetics,isolation & purification DNA, Single-Stranded Methanosarcina barkeri/enzymology,genetics Molecular Sequence Data Polymerase Chain Reaction Proton-Translocating ATPases/genetics,metabolism Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid

Chemicals

DNA, Bacterial DNA, Single-Stranded Proton-Translocating ATPases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Sumi M

Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Yokohama.

Sato M H

Denda K

Date T

Yoshida M

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/0014-5793(92)81472-x

Published

1992-12-21

Pages

207-10

Language

English

Region

England

NLM ID

0155157

Subset

Databases

GENBANK

D13551, D13552, D13553, S39996, S40001, S40008, S40009, S40010, S51589, X72580

External Links

PubMed source DOI: 10.1016/0014-5793(92)81472-x View journal details

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A DNA fragment homologous to F1-ATPase beta subunit was amplified from genomic DNA of Methanosarcina barkeri. Indication of an archaebacterial F-type ATPase.

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