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PMID: 14662743 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Yeast homotypic vacuole fusion requires the Ccz1-Mon1 complex during the tethering/docking stage.

The Journal of cell biology ·Vol. 163 ·No. 5 ·2003-12-08 ·Pages 973-85

Wang CW, Stromhaug PE, Kauffman EJ, Weisman LS, Klionsky DJ

Abstract

The function of the yeast lysosome/vacuole is critically linked with the morphology of the organelle. Accordingly, highly regulated processes control vacuolar fission and fusion events. Analysis of homotypic vacuole fusion demonstrated that vacuoles from strains defective in the CCZ1 and MON1 genes could not fuse. Morphological evidence suggested that these mutant vacuoles could not proceed to the tethering/docking stage. Ccz1 and Mon1 form a stable protein complex that binds the vacuole membrane. In the absence of the Ccz1-Mon1 complex, the integrity of vacuole SNARE pairing and the unpaired SNARE class C Vps/HOPS complex interaction were both impaired. The Ccz1-Mon1 complex colocalized with other fusion components on the vacuole as part of the cis-SNARE complex, and the association of the Ccz1-Mon1 complex with the vacuole appeared to be regulated by the class C Vps/HOPS complex proteins. Accordingly, we propose that the Ccz1-Mon1 complex is critical for the Ypt7-dependent tethering/docking stage leading to the formation of a trans-SNARE complex and subsequent vacuole fusion.

MeSH Terms
Biological Transport Carrier Proteins/genetics,metabolism Guanine Nucleotide Exchange Factors Macromolecular Substances Membrane Fusion/physiology Membrane Proteins/metabolism Recombinant Fusion Proteins/metabolism SNARE Proteins Saccharomyces cerevisiae/cytology,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Vacuoles/metabolism Vesicular Transport Proteins rab GTP-Binding Proteins/metabolism
Chemicals
Carrier Proteins Ccz1 protein, S cerevisiae Guanine Nucleotide Exchange Factors Macromolecular Substances Membrane Proteins Mon1 protein, S cerevisiae Recombinant Fusion Proteins SNARE Proteins Saccharomyces cerevisiae Proteins Vesicular Transport Proteins YPT7 protein, S cerevisiae rab GTP-Binding Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wang Chao-Wen
Life Sciences Institute, Department of Molecular, Cellular and Developmental Biology and Biological Chemistry, University of Michigan, Ann Arbor, MI 48109-2216, USA.
Stromhaug Per E
Kauffman Emily J
Weisman Lois S
Klionsky Daniel J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200308071
Published
2003-12-08
Pages
973-85
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC1705953
Subset
IM
Grants
NIGMS NIH HHS · R01 GM050403 · United States
NIGMS NIH HHS · R01 GM053396 · United States
NIGMS NIH HHS · GM50403 · United States
NIGMS NIH HHS · GM53396 · United States
External Links
PubMed source DOI: 10.1083/jcb.200308071 PMC Full Text View journal details
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