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PMID: 14657233 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Drosophila aPKC regulates cell polarity and cell proliferation in neuroblasts and epithelia.

The Journal of cell biology ·Vol. 163 ·No. 5 ·2003-12-08 ·Pages 1089-98

Rolls MM, Albertson R, Shih HP, Lee CY, Doe CQ

Abstract

Cell polarity is essential for generating cell diversity and for the proper function of most differentiated cell types. In many organisms, cell polarity is regulated by the atypical protein kinase C (aPKC), Bazooka (Baz/Par3), and Par6 proteins. Here, we show that Drosophila aPKC zygotic null mutants survive to mid-larval stages, where they exhibit defects in neuroblast and epithelial cell polarity. Mutant neuroblasts lack apical localization of Par6 and Lgl, and fail to exclude Miranda from the apical cortex; yet, they show normal apical crescents of Baz/Par3, Pins, Inscuteable, and Discs large and normal spindle orientation. Mutant imaginal disc epithelia have defects in apical/basal cell polarity and tissue morphology. In addition, we show that aPKC mutants show reduced cell proliferation in both neuroblasts and epithelia, the opposite of the lethal giant larvae (lgl) tumor suppressor phenotype, and that reduced aPKC levels strongly suppress most lgl cell polarity and overproliferation phenotypes.

MeSH Terms
Alleles Animals Carrier Proteins/metabolism Cell Cycle Proteins/metabolism Cell Division/physiology Cell Polarity Drosophila Proteins/genetics,metabolism Drosophila melanogaster/embryology,genetics,metabolism Epithelial Cells/cytology,physiology Intracellular Signaling Peptides and Proteins Larva/cytology,metabolism Neurons/cytology,physiology Phenotype Protein Kinase C/genetics,metabolism Tumor Suppressor Proteins
Chemicals
Carrier Proteins Cell Cycle Proteins Drosophila Proteins Intracellular Signaling Peptides and Proteins Mira protein, Drosophila Tumor Suppressor Proteins baz protein, Drosophila l(2)gl protein, Drosophila PKC-3 protein Protein Kinase C
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rolls Melissa M
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, 1254 University of Oregon, Eugene, OR 97403, USA.
Albertson Roger
Shih Hsin-Pei
Lee Cheng-Yu
Doe Chris Q
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200306079
Published
2003-12-08
Epub
2003-00-01
Pages
1089-98
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2173607
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200306079 PMC Full Text View journal details
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