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PMID: 14645290 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

InvB is a type III secretion-associated chaperone for the Salmonella enterica effector protein SopE.

Journal of bacteriology ·Vol. 185 ·No. 24 ·2003-12-00 ·Pages 7279-84

Lee SH, Galán JE

Abstract

SopE is a bacteriophage-encoded effector protein of Salmonella enterica serovar Typhimurium that is translocated into the cytosol of eukaryotic cells by a type III secretion system (TTSS) (W.-D. Hardt, H. Urlaub, and J. E. Galán, Proc. Natl. Acad. Sci. USA 95:2574-2579, 1998; M. W. Wood, R. Rosqvist, P. B. Mullan, M. H. Edwards, and E. E. Galyov, Mol. Microbiol. 22:327-338, 1996). In this study, we provide evidence that an unlinked gene carried within the Salmonella pathogenicity island 1 (SPI-1), invB (K. Eichelberg, C. Ginocchio, and J. E. Galán, J. Bacteriol. 176:4501-4510, 1994), is required for the secretion of SopE through the SPI-1 TTSS. Furthermore, far-Western blotting analysis shows that SopE directly interacts with InvB through a domain located at its amino terminus. We conclude that InvB is the TTSS-associated chaperone for SopE.

MeSH Terms
Bacterial Proteins/genetics,metabolism Blotting, Western Lac Operon Molecular Chaperones/genetics,metabolism Salmonella enterica/genetics,metabolism
Chemicals
Bacterial Proteins Molecular Chaperones SopE protein, Salmonella invB protein, Salmonella
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lee Sang Ho
Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut 06536, USA.
Galán Jorge E
References (34)
34 references, click to expand
  1. Assembly and function of type III secretory systems.
    Annu Rev Microbiol. 2000;54:735-74 PMID: 11018143
  2. InvB is a type III secretion chaperone specific for SspA.
    J Bacteriol. 2000 Dec;182(23):6638-44 PMID: 11073906
  3. A Salmonella inositol polyphosphatase acts in conjunction with other bacterial effectors to promote host cell actin cytoskeleton rearrangements and bacterial internalization.
    Mol Microbiol. 2001 Jan;39(2):248-59 PMID: 11136447
  4. Type III secretion chaperone-dependent regulation: activation of virulence genes by SicA and InvF in Salmonella typhimurium.
    EMBO J. 2001 Apr 17;20(8):1850-62 PMID: 11296219
  5. Salmonella interactions with host cells: type III secretion at work.
    Annu Rev Cell Dev Biol. 2001;17:53-86 PMID: 11687484
  6. Maintenance of an unfolded polypeptide by a cognate chaperone in bacterial type III secretion.
    Nature. 2001 Nov 1;414(6859):77-81 PMID: 11689946
  7. Three-dimensional secretion signals in chaperone-effector complexes of bacterial pathogens.
    Mol Cell. 2002 May;9(5):971-80 PMID: 12049734
  8. Structural basis for the reversible activation of a Rho protein by the bacterial toxin SopE.
    EMBO J. 2002 Jul 1;21(13):3286-95 PMID: 12093730
  9. Chaperones of the type III secretion pathway: jacks of all trades.
    Mol Microbiol. 2002 Oct;46(1):1-11 PMID: 12366826
  10. Mucosal priming of simian immunodeficiency virus-specific cytotoxic T-lymphocyte responses in rhesus macaques by the Salmonella type III secretion antigen delivery system.
    J Virol. 2003 Feb;77(4):2400-9 PMID: 12551977
  11. Priming virulence factors for delivery into the host.
    Nat Rev Mol Cell Biol. 2003 Sep;4(9):738-43 PMID: 14506477
  12. Cloning and molecular characterization of genes whose products allow Salmonella typhimurium to penetrate tissue culture cells.
    Proc Natl Acad Sci U S A. 1989 Aug;86(16):6383-7 PMID: 2548211
  13. SycE, a chaperone-like protein of Yersinia enterocolitica involved in Ohe secretion of YopE.
    Mol Microbiol. 1993 Apr;8(1):123-31 PMID: 8497188
  14. The adenovirus E4-6/7 protein transactivates the E2 promoter by inducing dimerization of a heteromeric E2F complex.
    Mol Cell Biol. 1994 Feb;14(2):1333-46 PMID: 8289811
  15. Molecular and functional characterization of the Salmonella typhimurium invasion genes invB and invC: homology of InvC to the F0F1 ATPase family of proteins.
    J Bacteriol. 1994 Aug;176(15):4501-10 PMID: 8045880
  16. Individual chaperones required for Yop secretion by Yersinia.
    Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10493-7 PMID: 7937981
  17. Extracellular association and cytoplasmic partitioning of the IpaB and IpaC invasins of S. flexneri.
    Cell. 1994 Nov 4;79(3):515-25 PMID: 7954817
  18. The Salmonella typhimurium invasion genes invF and invG encode homologues of the AraC and PulD family of proteins.
    Mol Microbiol. 1994 Aug;13(4):555-68 PMID: 7997169
  19. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.
    J Bacteriol. 1995 Jul;177(14):4121-30 PMID: 7608087
  20. Cell-surface-bound Yersinia translocate the protein tyrosine phosphatase YopH by a polarized mechanism into the target cell.
    Mol Microbiol. 1995 Oct;18(1):135-50 PMID: 8596454
  21. Customized secretion chaperones in pathogenic bacteria.
    Mol Microbiol. 1996 Apr;20(2):255-62 PMID: 8733225
  22. Salmonella spp. are cytotoxic for cultured macrophages.
    Mol Microbiol. 1996 Sep;21(5):1101-15 PMID: 8885278
  23. SopE, a secreted protein of Salmonella dublin, is translocated into the target eukaryotic cell via a sip-dependent mechanism and promotes bacterial entry.
    Mol Microbiol. 1996 Oct;22(2):327-38 PMID: 8930917
  24. A substrate of the centisome 63 type III protein secretion system of Salmonella typhimurium is encoded by a cryptic bacteriophage.
    Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2574-9 PMID: 9482928
  25. S. typhimurium encodes an activator of Rho GTPases that induces membrane ruffling and nuclear responses in host cells.
    Cell. 1998 May 29;93(5):815-26 PMID: 9630225
  26. Identification of a specific chaperone for SptP, a substrate of the centisome 63 type III secretion system of Salmonella typhimurium.
    J Bacteriol. 1998 Jul;180(13):3393-9 PMID: 9642193
  27. YscB of Yersinia pestis functions as a specific chaperone for YopN.
    J Bacteriol. 1998 Sep;180(18):4912-21 PMID: 9733695
  28. Type III secretion machines: bacterial devices for protein delivery into host cells.
    Science. 1999 May 21;284(5418):1322-8 PMID: 10334981
  29. Differential regulation of Salmonella typhimurium type III secreted proteins by pathogenicity island 1 (SPI-1)-encoded transcriptional activators InvF and hilA.
    Infect Immun. 1999 Aug;67(8):4099-105 PMID: 10417179
  30. Isolation of a temperate bacteriophage encoding the type III effector protein SopE from an epidemic Salmonella typhimurium strain.
    Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9845-50 PMID: 10449782
  31. Complex function for SicA, a Salmonella enterica serovar typhimurium type III secretion-associated chaperone.
    J Bacteriol. 2000 Apr;182(8):2262-8 PMID: 10735870
  32. Striking a balance: modulation of the actin cytoskeleton by Salmonella.
    Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8754-61 PMID: 10922031
  33. LcrQ/YscM1, regulators of the Yersinia yop virulon, are injected into host cells by a chaperone-dependent mechanism.
    Mol Microbiol. 2000 Jul;37(2):263-73 PMID: 10931323
  34. Identification of SopE2 from Salmonella typhimurium, a conserved guanine nucleotide exchange factor for Cdc42 of the host cell.
    Mol Microbiol. 2000 Jun;36(6):1206-21 PMID: 10931274
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.185.24.7279-7284.2003
Published
2003-12-00
Pages
7279-84
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC296260
Subset
IM
Grants
NIAID NIH HHS · R37 AI030492 · United States
NIAID NIH HHS · F32 AI052710 · United States
NIAID NIH HHS · AI52710-01 · United States
NIAID NIH HHS · AI30492 · United States
NIAID NIH HHS · R01 AI030492 · United States
External Links
PubMed source DOI: 10.1128/JB.185.24.7279-7284.2003 PMC Full Text View journal details
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