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PMID: 14631044 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Synchronization of cellular clocks in the suprachiasmatic nucleus.

Science (New York, N.Y.) ·Vol. 302 ·No. 5649 ·2003-11-21 ·Pages 1408-12

Yamaguchi S, Isejima H, Matsuo T, Okura R, Yagita K, Kobayashi M, Okamura H

Abstract

Individual cellular clocks in the suprachiasmatic nucleus (SCN), the circadian center, are integrated into a stable and robust pacemaker with a period length of about 24 hours. We used real-time analysis of gene expression to show synchronized rhythms of clock gene transcription across hundreds of neurons within the mammalian SCN in organotypic slice culture. Differentially phased neuronal clocks are topographically arranged across the SCN. A protein synthesis inhibitor set all cell clocks to the same initial phase and, after withdrawal, intrinsic interactions among cell clocks reestablished the stable program of gene expression across the assemblage. Na+-dependent action potentials contributed to establishing cellular synchrony and maintaining spontaneous oscillation across the SCN.

MeSH Terms

ARNTL Transcription Factors Action Potentials/drug effects Animals Basic Helix-Loop-Helix Transcription Factors Biological Clocks/physiology CLOCK Proteins Cell Cycle Proteins Circadian Rhythm/physiology Cycloheximide/pharmacology Gene Expression Glyceraldehyde-3-Phosphate Dehydrogenases/genetics,metabolism Luminescence Mice Mice, Knockout Mice, Transgenic Neurons/physiology Nuclear Proteins/genetics,metabolism Organ Culture Techniques Period Circadian Proteins Promoter Regions, Genetic Protein Synthesis Inhibitors/pharmacology Signal Transduction Sodium/metabolism Suppression, Genetic Suprachiasmatic Nucleus/cytology,physiology Tetrodotoxin/pharmacology Trans-Activators/metabolism Transcription Factors/metabolism Transcription, Genetic

Chemicals

ARNTL Transcription Factors Arntl protein, mouse Basic Helix-Loop-Helix Transcription Factors Cell Cycle Proteins Nuclear Proteins Per1 protein, mouse Per2 protein, mouse Period Circadian Proteins Protein Synthesis Inhibitors Trans-Activators Transcription Factors Tetrodotoxin Cycloheximide Sodium Glyceraldehyde-3-Phosphate Dehydrogenases CLOCK Proteins Clock protein, mouse

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Yamaguchi Shun

Division of Molecular Brain Science, Department of Brain Sciences, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.

Isejima Hiromi

Matsuo Takuya

Okura Ryusuke

Yagita Kazuhiro

Kobayashi Masaki

Okamura Hitoshi

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

1095-9203

DOI

10.1126/science.1089287

Published

2003-11-21

Pages

1408-12

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.1089287 View journal details

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Synchronization of cellular clocks in the suprachiasmatic nucleus.

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