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PMID: 14610052 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Morphologically distinct microtubule ends in the mitotic centrosome of Caenorhabditis elegans.

The Journal of cell biology ·Vol. 163 ·No. 3 ·2003-11-10 ·Pages 451-6

O'Toole ET, McDonald KL, Mäntler J, McIntosh JR, Hyman AA, Müller-Reichert T

Abstract

During mitosis, the connections of microtubules (MTs) to centrosomes and kinetochores are dynamic. From in vitro studies, it is known that the dynamic behavior of MTs is related to the structure of their ends, but we know little about the structure of MT ends in spindles. Here, we use high-voltage electron tomography to study the centrosome- and kinetochore-associated ends of spindle MTs in embryonic cells of the nematode, Caenorhabditis elegans. Centrosome-associated MT ends are either closed or open. Closed MT ends are more numerous and are uniformly distributed around the centrosome, but open ends are found preferentially on kinetochore-attached MTs. These results have structural implications for models of MT interactions with centrosomes.

MeSH Terms
Animals Caenorhabditis elegans/metabolism,ultrastructure Centrioles/physiology,ultrastructure Centrosome/physiology,ultrastructure Chromatin/physiology,ultrastructure Kinetochores/physiology,ultrastructure Microtubules/metabolism,ultrastructure Mitosis/physiology Spindle Apparatus/physiology,ultrastructure Tomography, X-Ray Computed
Chemicals
Chromatin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
O'Toole Eileen T
Boulder Laboratory for 3-D Electron Microscopy of Cells, University of Colorado, 80309, USA.
McDonald Kent L
Mäntler Jana
McIntosh J Richard
Hyman Anthony A
Müller-Reichert Thomas
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200304035
Published
2003-11-10
Pages
451-6
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2173630
Subset
IM
Grants
NCRR NIH HHS · P41 RR000592 · United States
NCRR NIH HHS · RR00592 · United States
External Links
PubMed source DOI: 10.1083/jcb.200304035 PMC Full Text View journal details
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