Abstract
The medicinal value associated with complex polyketide and nonribosomal peptide natural products has prompted biosynthetic schemes dependent upon heterologous microbial hosts. Here we report the successful biosynthesis of yersiniabactin (Ybt), a model polyketide-nonribosomal peptide hybrid natural product, using Escherichia coli as a heterologous host. After introducing the biochemical pathway for Ybt into E. coli, biosynthesis was initially monitored qualitatively by mass spectrometry. Next, production of Ybt was quantified in a high-cell-density fermentation environment with titers reaching 67 +/- 21 (mean +/- standard deviation) mg/liter and a volumetric productivity of 1.1 +/- 0.3 mg/liter-h. This success has implications for basic and applied studies on Ybt biosynthesis and also, more generally, for future production of polyketide, nonribosomal peptide, and mixed polyketide-nonribosomal peptide natural products using E. coli.
MeSH Terms
Bacterial Outer Membrane Proteins
Bacterial Proteins/genetics,metabolism
Biotechnology/methods
Escherichia coli/genetics,growth & development,metabolism
Fermentation
Iron-Binding Proteins
Mass Spectrometry
Periplasmic Binding Proteins
Phenols
Siderophores/biosynthesis
Thiazoles
Chemicals
Bacterial Outer Membrane Proteins
Bacterial Proteins
Iron-Binding Proteins
Periplasmic Binding Proteins
Phenols
Siderophores
Thiazoles
yersiniabactin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pfeifer Blaine A
Chemical Engineering, Stanford University, Stanford, California 94305, USA.
Wang Clay C C
Walsh Christopher T
Khosla Chaitan
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