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PMID: 14602078 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Coactivation of STAT and Ras is required for germ cell proliferation and invasive migration in Drosophila.

Developmental cell ·Vol. 5 ·No. 5 ·2003-11-00 ·Pages 787-98

Li J, Xia F, Li WX

Abstract

Primordial germ cells (PGCs) undergo proliferation, invasion, guided migration, and aggregation to form the gonad. Here we show that in Drosophila, the receptor tyrosine kinase Torso activates both STAT and Ras during the early phase of PGC development, and coactivation of STAT and Ras is required for PGC proliferation and invasive migration. Embryos mutant for stat92E or Ras1 have fewer PGCs, and these cells migrate slowly, errantly, and fail to coalesce. Conversely, overactivation of these molecules causes supernumerary PGCs, their premature transit through the gut epithelium, and ectopic colonization. A requirement for RTK in Drosophila PGC development is analogous to the mouse, in which the RTK c-kit is required, suggesting a conserved molecular mechanism governing PGC behavior in flies and mammals.

MeSH Terms
Animals Cell Division/physiology Cell Movement/physiology DNA-Binding Proteins/metabolism Drosophila Proteins/genetics,metabolism Drosophila melanogaster/anatomy & histology,physiology Embryo, Nonmammalian/anatomy & histology,physiology Enzyme Activation Evolution, Molecular Female GTP-Binding Proteins/metabolism Germ Cells/cytology,physiology Janus Kinases Male Mice Mitosis/physiology Mutation Protein-Tyrosine Kinases/genetics,metabolism Receptor Protein-Tyrosine Kinases/genetics,metabolism STAT Transcription Factors Signal Transduction/physiology Trans-Activators/metabolism Transcription Factors ras Proteins
Chemicals
DNA-Binding Proteins Drosophila Proteins STAT Transcription Factors Stat92E protein, Drosophila Trans-Activators Transcription Factors Protein-Tyrosine Kinases Receptor Protein-Tyrosine Kinases tor protein, Drosophila Janus Kinases hop protein, Drosophila GTP-Binding Proteins Ras85D protein, Drosophila ras Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Li Jinghong
Department of Biomedical Genetics, University of Rochester Medical Center, Rochester, NY 14642, USA.
Xia Fan
Li Willis X
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Article Info
Journal
Developmental cell
Abbr.
Dev Cell
ISSN
1534-5807
DOI
10.1016/s1534-5807(03)00328-9
Published
2003-11-00
Pages
787-98
Language
English
Region
United States
NLM ID
101120028
PMCID
PMC3092433
Subset
IM
Grants
NIGMS NIH HHS · R01 GM065774 · United States
NIGMS NIH HHS · R01 GM065774-02 · United States
NIGMS NIH HHS · R01 GM65774 · United States
External Links
PubMed source DOI: 10.1016/s1534-5807(03)00328-9 PMC Full Text View journal details
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