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PMID: 14582188 Published · ppublish English Journal Article

New properties of simian virus 40 large T antigen.

Molecular and cellular biology ·Vol. 2 ·No. 12 ·1982-12-00 ·Pages 1463-71

Seif R

Abstract

An 8,000-molecular-weight (8K) T antigen was found in all cells transformed by simian virus 40. The 8K T antigen was weakly labeled in vivo with [35S]methionine or 32Pi. A deletion in the human papovavirus BK genome, in the region coding for the carboxy-terminal end of the large T antigen, reduced the size of the 8K T antigen. The last 80 amino acids of the large T antigen include the sequence Asp-Asp-Asp-Asp unique to the activation peptide of trypsinogen. Large T antigen bound diisopropyl fluorophosphate and was retained by D-phenylalanine coupled to Sepharose beads, an affinity adsorbent that can retain chymotrypsin. The large T antigen and the recA protein of Escherichia coli, a known protease, have several properties in common as well as several similar sequences. Antibodies against large T antigen interacted with native recA protein.

MeSH Terms
Amino Acid Sequence Animals Antigens, Polyomavirus Transforming/chemistry,genetics,metabolism BK Virus/genetics Cell Line, Transformed Cell Transformation, Viral Humans Isoflurophate/metabolism Molecular Sequence Data Molecular Weight Protease Inhibitors/metabolism Protein Binding Rats Simian virus 40/physiology
Chemicals
Antigens, Polyomavirus Transforming Protease Inhibitors Isoflurophate
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Seif R
Laboratoire d'Enzymologie, Centre National de la Recherche Scientifique, 91190 Gif-sur-Yvette, France.
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.2.12.1463-1471.1982
Published
1982-12-00
Pages
1463-71
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369955
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.2.12.1463-1471.1982 PMC Full Text View journal details
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