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PMID: 14561880 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Coincident Hfq binding and RNase E cleavage sites on mRNA and small regulatory RNAs.

RNA (New York, N.Y.) ·Vol. 9 ·No. 11 ·2003-11-00 ·Pages 1308-14

Moll I, Afonyushkin T, Vytvytska O, Kaberdin VR, Bläsi U

Abstract

The Escherichia coli RNA chaperone Hfq was discovered originally as an accessory factor of the phage Qbeta replicase. More recent work suggested a role of Hfq in cellular physiology through its interaction with ompA mRNA and small RNAs (sRNAs), some of which are involved in translational regulation. Despite their stability under certain conditions, E. coli sRNAs contain putative RNase E recognition sites, that is, A/U-rich sequences and adjacent stem-loop structures. We show herein that an RNase E cleavage site coincides with the Hfq-binding site in the 5'-untranslated region of E. coli ompA mRNA as well as with that in the sRNA, DsrA. Likewise, Hfq protects RyhB RNA from in vitro cleavage by RNase E. These in vitro data are supported by the increased abundance of DsrA and RyhB sRNAs in an RNase E mutant strain as well as by their decreased stability in a hfq(-) strain. It is commonly believed that the RNA chaperone Hfq facilitates or promotes the interaction between sRNAs and their mRNA targets. This study reveals another role for Hfq, that is, protection of sRNAs from endonucleolytic attack.

MeSH Terms
5' Untranslated Regions Base Sequence Binding Sites Endoribonucleases/metabolism Host Factor 1 Protein/metabolism Hydrolysis Molecular Sequence Data Nucleic Acid Conformation Promoter Regions, Genetic RNA, Messenger/chemistry,metabolism
Chemicals
5' Untranslated Regions Host Factor 1 Protein RNA, Messenger Endoribonucleases ribonuclease E
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Moll Isabella
Department of Microbiology and Genetics, University Departments at the Vienna Biocenter, Dr Bohrgasse 9, 1030 Vienna, Austria. ISA@GEM.UNIVIE.AC.AT
Afonyushkin Taras
Vytvytska Oresta
Kaberdin Vladimir R
Bläsi Udo
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35 references, click to expand
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1261/rna.5850703
Email
isa@gem.univie.ac.at
Published
2003-11-00
Pages
1308-14
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1287052
Subset
IM
External Links
PubMed source DOI: 10.1261/rna.5850703 PMC Full Text View journal details
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